PMID- 23664188
OWN - NLM
STAT- MEDLINE
DCOM- 20140121
LR  - 20220129
IS  - 1873-569X (Electronic)
IS  - 0923-1811 (Linking)
VI  - 71
IP  - 1
DP  - 2013 Jul
TI  - Invariant natural killer T cells are enriched at the site of cutaneous 
      inflammation in lupus erythematosus.
PG  - 22-8
LID - S0923-1811(13)00127-8 [pii]
LID - 10.1016/j.jdermsci.2013.04.012 [doi]
AB  - BACKGROUND: Systemic lupus erythematosus (SLE) is associated with a numerical and 
      functional reduction of peripheral blood (PB) invariant natural killer T (iNKT) 
      cells. Limited information exists on the role of iNKT cells in the pathogenesis 
      of lupus erythematosus. OBJECTIVE: To investigate the frequency and phenotype of 
      iNKT cells in PB and dermal infiltrates from patients with SLE, 
      subacute-cutaneous lupus erythematosus (SCLE) and discoid lupus erythematosus 
      (DLE). METHODS: PB was obtained from 23 SLE, 6 SCLE, and 11 DLE patients, and 
      from 30 healthy controls. iNKT cell frequency and CCR4/CCR6 surface expression 
      were assessed by flow cytometry. The frequency and phenotype of skin infiltrating 
      Valpha24(+)Vbeta11(+) iNKT cells were investigated by immunofluorescence in lesional 
      biopsies from 20 patients, unaffected skin from 3 patients, and from 6 healthy 
      controls. RESULTS: Lupus erythematosus patients displayed significantly lower 
      percentages of circulating CD3(+)6B11(+) iNKT cells compared to healthy controls. 
      Whereas CCR6 expression on iNKT cells was enhanced in active SLE patients 
      regardless of cutaneous involvement compared to healthy controls, CCR4 was 
      exclusively increased in patients with active cutaneous lesions. Furthermore, 
      iNKT cells were significantly enriched in lesional skin of SLE and DLE patients, 
      but not in unaffected skin of lupus patients. The majority of lesional iNKT cells 
      expressed IFN-gamma and CCR4. CONCLUSION: The deficiency in circulating iNKT cells in 
      cutaneous lupus erythematosus is associated with an increase of iNKT cells at the 
      site of cutaneous inflammation. These data underscore the importance of analyzing 
      iNKT cells not only in PB, but also in the target tissues.
CI  - Copyright (c) 2013 Japanese Society for Investigative Dermatology. Published by 
      Elsevier Ireland Ltd. All rights reserved.
FAU - Hofmann, Silke C
AU  - Hofmann SC
AD  - Centre for Rheumatology Research, University College London, UK. 
      silke.hofmann@helios-kliniken.de
FAU - Bosma, Anneleen
AU  - Bosma A
FAU - Bruckner-Tuderman, Leena
AU  - Bruckner-Tuderman L
FAU - Vukmanovic-Stejic, Milica
AU  - Vukmanovic-Stejic M
FAU - Jury, Elizabeth C
AU  - Jury EC
FAU - Isenberg, David A
AU  - Isenberg DA
FAU - Mauri, Claudia
AU  - Mauri C
LA  - eng
GR  - 18106/ARC_/Arthritis Research UK/United Kingdom
GR  - 19314/VAC_/Versus Arthritis/United Kingdom
GR  - 17707/ARC_/Arthritis Research UK/United Kingdom
GR  - 18106/VAC_/Versus Arthritis/United Kingdom
GR  - 20085/ARC_/Arthritis Research UK/United Kingdom
GR  - 17707/VAC_/Versus Arthritis/United Kingdom
GR  - 19314/ARC_/Arthritis Research UK/United Kingdom
GR  - G0901102/MRC_/Medical Research Council/United Kingdom
GR  - 20085/VAC_/Versus Arthritis/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130419
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (Biomarkers)
RN  - 0 (CCR4 protein, human)
RN  - 0 (CCR6 protein, human)
RN  - 0 (CD3 Complex)
RN  - 0 (IFNG protein, human)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 0 (Receptors, CCR4)
RN  - 0 (Receptors, CCR6)
RN  - 0 (Valpha24 protein, human)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biomarkers/analysis
MH  - Biopsy
MH  - CD3 Complex/analysis
MH  - Case-Control Studies
MH  - Female
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Immunophenotyping/methods
MH  - Inflammation Mediators/analysis
MH  - Interferon-gamma/analysis
MH  - Lupus Erythematosus, Cutaneous/*immunology/pathology
MH  - Lupus Erythematosus, Discoid/immunology/pathology
MH  - Lupus Erythematosus, Systemic/*immunology/pathology
MH  - Male
MH  - Middle Aged
MH  - Natural Killer T-Cells/*immunology
MH  - Phenotype
MH  - Prospective Studies
MH  - Receptors, Antigen, T-Cell/analysis
MH  - Receptors, Antigen, T-Cell, alpha-beta/analysis
MH  - Receptors, CCR4/analysis
MH  - Receptors, CCR6/analysis
MH  - Skin/*immunology/pathology
MH  - Young Adult
EDAT- 2013/05/15 06:00
MHDA- 2014/01/22 06:00
CRDT- 2013/05/14 06:00
PHST- 2012/09/15 00:00 [received]
PHST- 2013/03/03 00:00 [revised]
PHST- 2013/04/04 00:00 [accepted]
PHST- 2013/05/14 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2014/01/22 06:00 [medline]
AID - S0923-1811(13)00127-8 [pii]
AID - 10.1016/j.jdermsci.2013.04.012 [doi]
PST - ppublish
SO  - J Dermatol Sci. 2013 Jul;71(1):22-8. doi: 10.1016/j.jdermsci.2013.04.012. Epub 
      2013 Apr 19.