PMID- 23665206
OWN - NLM
STAT- MEDLINE
DCOM- 20130820
LR  - 20130607
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 282
IP  - 1
DP  - 2013 Mar
TI  - Effects of tolerogenic dendritic cells generated by siRNA-mediated RelB silencing 
      on immune defense and surveillance functions of T cells.
PG  - 28-37
LID - S0008-8749(13)00040-3 [pii]
LID - 10.1016/j.cellimm.2013.03.004 [doi]
AB  - Dendritic cells (DCs) can initiate immune responses or induce immune tolerance, 
      according to their level of maturation. In this study, we inhibited RelB 
      expression in immature (im)DCs using small interfering RNA (siRNA) to maintain 
      their immature status. RelB-silenced DCs induced donor-specific 
      hyporesponsiveness in T cells. In contrast, T cells primed by RelB-silenced DCs 
      maintained normal proliferation and cytokine secretion when stimulated by 
      influenza virus antigen. Proliferation was similar between T cells stimulated 
      with syngeneic tumor antigen or donor-specific antigen stimulation, but was 
      significantly lower compared with T cells stimulated with influenza virus 
      antigen. Moreover, an altered pattern of micro181a and micro155 transcriptional 
      levels in T cells was involved in the differential regulation. This study 
      demonstrates that RelB-silenced DCs could induce donor-specific 
      hyporesponsiveness and slightly impair immune surveillance of T cells, while 
      retaining their immune defense functions.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Luo, Lei
AU  - Luo L
AD  - Department of Research and Education, Guizhou Province People's Hospital, Guiyang 
      550002, China. luolei7710@yahoo.com.cn
FAU - Sun, Zhaolin
AU  - Sun Z
FAU - Fang, Qian
AU  - Fang Q
FAU - Huang, Shan
AU  - Huang S
FAU - Bai, Xiaoling
AU  - Bai X
FAU - Luo, Guangheng
AU  - Luo G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130404
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn155 microRNA, mouse)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Relb protein, mouse)
RN  - 147337-75-5 (Transcription Factor RelB)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/metabolism
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Immune Tolerance/genetics/immunology
MH  - Lymphocyte Culture Test, Mixed/methods
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/genetics/immunology
MH  - Microscopy, Confocal
MH  - RNA Interference/*immunology
MH  - RNA, Small Interfering/genetics/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes/*immunology/metabolism
MH  - Transcription Factor RelB/genetics/*immunology/metabolism
EDAT- 2013/05/15 06:00
MHDA- 2013/08/21 06:00
CRDT- 2013/05/14 06:00
PHST- 2012/12/12 00:00 [received]
PHST- 2013/02/05 00:00 [revised]
PHST- 2013/03/26 00:00 [accepted]
PHST- 2013/05/14 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2013/08/21 06:00 [medline]
AID - S0008-8749(13)00040-3 [pii]
AID - 10.1016/j.cellimm.2013.03.004 [doi]
PST - ppublish
SO  - Cell Immunol. 2013 Mar;282(1):28-37. doi: 10.1016/j.cellimm.2013.03.004. Epub 
      2013 Apr 4.