PMID- 23665235
OWN - NLM
STAT- MEDLINE
DCOM- 20140211
LR  - 20210526
IS  - 1878-5875 (Electronic)
IS  - 1357-2725 (Linking)
VI  - 45
IP  - 8
DP  - 2013 Aug
TI  - MiR-224 impairs adipocyte early differentiation and regulates fatty acid 
      metabolism.
PG  - 1585-93
LID - S1357-2725(13)00134-9 [pii]
LID - 10.1016/j.biocel.2013.04.029 [doi]
AB  - MicroRNAs (miRNAs) are small ~22 nucleotide regulatory RNAs that regulate the 
      stability and translation of cognate messenger RNAs (mRNAs). MicroRNAs 
      participate in the regulation of adipogenesis and identification of the full 
      repertoire of MicroRNAs expressed in adipose tissue is likely to improve our 
      understanding of adipose tissue growth and development significantly. In the 
      present study, it is found that miR-224-5p abundance decreases first and then 
      increases during adipogenesis of 3T3-L1 cells. And early growth response 2 (EGR2) 
      and Acyl-CoA synthetase long-chain family member 4 (ACSL4) are direct targets of 
      miR-224-5p. Further studies in mouse 3T3-L1 cell-line shows that miR-224-5p is a 
      novel negative regulator of adipocyte differentiation through 
      post-transcriptional regulation of early growth response 2 during early 
      adipogenesis. Furthermore, miR-224-5p could regulate fatty acid metabolism 
      through Acyl-CoA synthetase long-chain family member 4 at terminal 
      differentiation. It indicates that miR-224 plays different roles on different 
      stages of adipogenesis.
CI  - Crown Copyright (c) 2013. Published by Elsevier Ltd. All rights reserved.
FAU - Peng, Yongdong
AU  - Peng Y
AD  - Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College 
      of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 
      People's Republic of China. pyongdong123@163.com
FAU - Xiang, Hong
AU  - Xiang H
FAU - Chen, Chen
AU  - Chen C
FAU - Zheng, Rong
AU  - Zheng R
FAU - Chai, Jin
AU  - Chai J
FAU - Peng, Jian
AU  - Peng J
FAU - Jiang, Siwen
AU  - Jiang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130510
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Early Growth Response Protein 2)
RN  - 0 (Egr2 protein, mouse)
RN  - 0 (Fatty Acids)
RN  - 0 (MIRN224 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - EC 6.2.1.- (Acsl4 protein, mouse)
RN  - EC 6.2.1.- (Coenzyme A Ligases)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - 3T3-L1 Cells
MH  - Adipocytes/*cytology/*metabolism
MH  - Adipogenesis/genetics
MH  - Animals
MH  - Base Sequence
MH  - Cell Differentiation/*genetics
MH  - Coenzyme A Ligases/metabolism
MH  - Conserved Sequence/genetics
MH  - Early Growth Response Protein 2/metabolism
MH  - Fatty Acids/*metabolism
MH  - Gene Expression Regulation
MH  - Lipid Metabolism/genetics
MH  - Mammals/genetics
MH  - Mice
MH  - MicroRNAs/genetics/*metabolism
MH  - Molecular Sequence Data
OTO - NOTNLM
OT  - ACSL4
OT  - Adipogenesis
OT  - EGR2
OT  - Fatty acid metabolism
OT  - MiR-224
EDAT- 2013/05/15 06:00
MHDA- 2014/02/12 06:00
CRDT- 2013/05/14 06:00
PHST- 2013/04/03 00:00 [received]
PHST- 2013/04/23 00:00 [revised]
PHST- 2013/04/29 00:00 [accepted]
PHST- 2013/05/14 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2014/02/12 06:00 [medline]
AID - S1357-2725(13)00134-9 [pii]
AID - 10.1016/j.biocel.2013.04.029 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2013 Aug;45(8):1585-93. doi: 
      10.1016/j.biocel.2013.04.029. Epub 2013 May 10.