PMID- 23665278
OWN - NLM
STAT- MEDLINE
DCOM- 20140109
LR  - 20141120
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 158
IP  - 1
DP  - 2013 Jun
TI  - Effects of steroid hormones on reproduction- and detoxification-related gene 
      expression in adult male mosquitofish, Gambusia affinis.
PG  - 36-43
LID - S1532-0456(13)00051-3 [pii]
LID - 10.1016/j.cbpc.2013.05.001 [doi]
AB  - The molecular mechanisms that mediate fish reproduction and detoxification in 
      response to steroid hormones were studied by using adult male western 
      mosquitofish (Gambusia affinis) as sentinel species. The expression patterns of 
      three vitellogenins (VtgA, VtgB and VtgC), two estrogen receptors (ERalpha and ERbeta), 
      two androgen receptors (ARalpha and ARbeta), metallothionein (MT) and cytochrome P450 1A 
      (CYP1A) in the liver and testis of adult male mosquitofish were assessed through 
      exposure treatments with progesterone (P), testosterone (T) and 17beta-estradiol 
      (E2), alone and in combination for eight days. The results showed that expression 
      patterns of Vtg subtype, ER subtype, AR subtype, MT and CYP1A genes in male 
      mosquitofish varied according to tissue and specific hormone stress. Vtg subtype 
      mRNA expression was induced in the liver in E2-added treatments, and an 
      up-regulation of ERalpha mRNA expression was also observed. In addition, hormone 
      treatments increased three Vtg subtype mRNA expression levels in the testis, at 
      least to some extent. All hormone treatments significantly inhibited ERalpha, ERbeta and 
      ARbeta mRNA expression in the testis. Some of hormone treatments could affect MT and 
      CYP1A gene expression in mosquitofish. In general, multiple hormone treatments 
      showed different effects on target gene expression compared with corresponding 
      hormone alone. The results from the present study provided valuable information 
      on the toxicological effects of steroid hormones in mosquitofish.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Huang, Guo-Yong
AU  - Huang GY
AD  - State Key Laboratory of Organic Geochemistry, Guangzhou Institute of 
      Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. 
      huang_gyh@sina.com
FAU - Ying, Guang-Guo
AU  - Ying GG
FAU - Liang, Yan-Qiu
AU  - Liang YQ
FAU - Liu, You-Sheng
AU  - Liu YS
FAU - Liu, Shuang-Shuang
AU  - Liu SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130507
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Vitellogenins)
RN  - 3XMK78S47O (Testosterone)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - 9038-94-2 (Metallothionein)
SB  - IM
MH  - Animals
MH  - Cyprinodontiformes/genetics/metabolism/*physiology
MH  - Cytochrome P-450 Enzyme System/genetics
MH  - Estradiol/pharmacology/*toxicity
MH  - Gene Expression/*drug effects/genetics
MH  - Inactivation, Metabolic
MH  - Liver/drug effects/metabolism
MH  - Male
MH  - Metallothionein/biosynthesis/genetics
MH  - Progesterone/pharmacology/*toxicity
MH  - RNA, Messenger/genetics
MH  - Receptors, Androgen/biosynthesis/genetics
MH  - Receptors, Estrogen/biosynthesis/genetics
MH  - Reproduction/*drug effects/genetics
MH  - Testis/drug effects/metabolism
MH  - Testosterone/pharmacology/*toxicity
MH  - Up-Regulation/drug effects
MH  - Vitellogenins/biosynthesis/genetics
EDAT- 2013/05/15 06:00
MHDA- 2014/01/10 06:00
CRDT- 2013/05/14 06:00
PHST- 2013/03/24 00:00 [received]
PHST- 2013/05/02 00:00 [revised]
PHST- 2013/05/02 00:00 [accepted]
PHST- 2013/05/14 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2014/01/10 06:00 [medline]
AID - S1532-0456(13)00051-3 [pii]
AID - 10.1016/j.cbpc.2013.05.001 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2013 Jun;158(1):36-43. doi: 
      10.1016/j.cbpc.2013.05.001. Epub 2013 May 7.