PMID- 23665627
OWN - NLM
STAT- MEDLINE
DCOM- 20131017
LR  - 20190827
IS  - 1348-2246 (Electronic)
IS  - 0910-6340 (Linking)
VI  - 29
IP  - 5
DP  - 2013
TI  - Optimization of an online post-column derivatization system for ultra 
      high-performance liquid chromatography (UHPLC) and its applications to analysis 
      of biogenic amines.
PG  - 539-45
AB  - We investigated the efficacy and utility of a post-column derivatization method 
      for the detection of amines with o-phthalaldehyde (OPA) in ultra high-performance 
      liquid chromatography (UHPLC). Since it was anticipated that a derivatization 
      reaction system placed downstream of the column would give considerable band 
      broadening to narrow peaks generated by a UHPLC column, we examined the 
      contributions of the dimensions of the reaction tube, the fluorescence (FL) 
      detector flow cell, and the column to the extra-column peak broadening, and 
      optimized the post-column derivatization system in UHPLC. We successfully 
      separated amines within 6 min with gradient elution by using the post-column 
      derivatization system connected to a UHPLC system. The analysis time was reduced 
      by a factor of 7 compared with that by conventional HPLC. The detection limits 
      were 18.0 - 105.7 fmol, depending on the compounds; the reproducibilities were 
      0.11 - 0.82% RSD for the retention times and 0.78 - 1.66% RSD for the peak areas 
      (n = 10), respectively. The linear dynamic ranges were up to 12, 20, and 40 
      pmol/muL, depending on the compounds, respectively.
FAU - Iijima, Satoe
AU  - Iijima S
AD  - LC and SFC Applications Laboratory, JASCO Corporation, 2967-5, Ishikawamachi, 
      Hachioji, Tokyo 192-8537, Japan. satoe.iijima@jasco.co.jp
FAU - Sato, Yasuyo
AU  - Sato Y
FAU - Bounoshita, Masao
AU  - Bounoshita M
FAU - Miyaji, Toshihiko
AU  - Miyaji T
FAU - Tognarelli, David J
AU  - Tognarelli DJ
FAU - Saito, Muneo
AU  - Saito M
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Anal Sci
JT  - Analytical sciences : the international journal of the Japan Society for 
      Analytical Chemistry
JID - 8511078
RN  - 0 (Biogenic Amines)
SB  - IM
MH  - Biogenic Amines/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Food Analysis
EDAT- 2013/05/15 06:00
MHDA- 2013/10/18 06:00
CRDT- 2013/05/14 06:00
PHST- 2013/05/14 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2013/10/18 06:00 [medline]
AID - DN/JST.JSTAGE/analsci/29.539 [pii]
AID - 10.2116/analsci.29.539 [doi]
PST - ppublish
SO  - Anal Sci. 2013;29(5):539-45. doi: 10.2116/analsci.29.539.