PMID- 23667177
OWN - NLM
STAT- MEDLINE
DCOM- 20140425
LR  - 20211021
IS  - 1539-7262 (Electronic)
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 54
IP  - 7
DP  - 2013 Jul
TI  - MCP-1 binds to oxidized LDL and is carried by lipoprotein(a) in human plasma.
PG  - 1877-83
LID - 10.1194/jlr.M036343 [doi]
AB  - Lipoprotein oxidation plays an important role in pathogenesis of atherosclerosis. 
      Oxidized low density lipoprotein (OxLDL) induces profound inflammatory responses 
      in vascular cells, such as production of monocyte chemoattractant protein-1 
      (MCP-1) [chemokine (C-C motif) ligand 2], a key chemokine in the initiation and 
      progression of vascular inflammation. Here we demonstrate that OxLDL also binds 
      MCP-1 and that the OxLDL-bound MCP-1 retains its ability to recruit monocytes. A 
      human MCP-1 mutant in which basic amino acids Arg-18 and Lys-19 were replaced 
      with Ala did not bind to OxLDL. The MCP-1 binding to OxLDL was inhibited by the 
      monoclonal antibody E06, which binds oxidized phospholipids (OxPLs) in OxLDL. 
      Because OxPLs are carried by lipoprotein(a) [Lp(a)] in human plasma, we tested to 
      determine whether Lp(a) binds MCP-1. Recombinant wild-type but not mutant MCP-1 
      added to human plasma bound to Lp(a), and its binding was inhibited by E06. Lp(a) 
      captured from human plasma contained MCP-1 and the Lp(a)-associated endogenous 
      MCP-1 induced monocyte migration. These results demonstrate that OxLDL and Lp(a) 
      bind MCP-1 in vitro and in vivo and that OxPLs are major determinants of the 
      MCP-1 binding. The association of MCP-1 with OxLDL and Lp(a) may play a role in 
      modulating monocyte trafficking during atherogenesis.
FAU - Wiesner, Philipp
AU  - Wiesner P
AD  - Department of Medicine, University of California, San Diego, San Diego, CA, USA.
FAU - Tafelmeier, Maria
AU  - Tafelmeier M
FAU - Chittka, Dominik
AU  - Chittka D
FAU - Choi, Soo-Ho
AU  - Choi SH
FAU - Zhang, Li
AU  - Zhang L
FAU - Byun, Young Sup
AU  - Byun YS
FAU - Almazan, Felicidad
AU  - Almazan F
FAU - Yang, Xiaohong
AU  - Yang X
FAU - Iqbal, Navaid
AU  - Iqbal N
FAU - Chowdhury, Punam
AU  - Chowdhury P
FAU - Maisel, Alan
AU  - Maisel A
FAU - Witztum, Joseph L
AU  - Witztum JL
FAU - Handel, Tracy M
AU  - Handel TM
FAU - Tsimikas, Sotirios
AU  - Tsimikas S
FAU - Miller, Yury I
AU  - Miller YI
LA  - eng
GR  - P01 HL088093/HL/NHLBI NIH HHS/United States
GR  - HL-055798/HL/NHLBI NIH HHS/United States
GR  - HL-088093/HL/NHLBI NIH HHS/United States
GR  - AI-037113/AI/NIAID NIH HHS/United States
GR  - R01 AI037113/AI/NIAID NIH HHS/United States
GR  - P01 HL055798/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130510
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipoprotein(a))
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Recombinant Proteins)
RN  - 0 (oxidized low density lipoprotein)
SB  - IM
EIN - J Lipid Res. 2014 Jul;55(7):1549. PMID: 24982241
MH  - Animals
MH  - Binding Sites
MH  - Chemokine CCL2/blood/*metabolism
MH  - Humans
MH  - Lipoprotein(a)/*blood/*metabolism
MH  - Lipoproteins, LDL/blood/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Recombinant Proteins/blood/metabolism
PMC - PMC3679389
OTO - NOTNLM
OT  - chemokine (C-C motif) ligand 2
OT  - monocyte chemoattractant protein-1
OT  - monocyte migration
OT  - oxidized low density lipoprotein
EDAT- 2013/05/15 06:00
MHDA- 2014/04/26 06:00
PMCR- 2014/07/01
CRDT- 2013/05/14 06:00
PHST- 2013/05/14 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2014/04/26 06:00 [medline]
PHST- 2014/07/01 00:00 [pmc-release]
AID - S0022-2275(20)42135-2 [pii]
AID - m036343 [pii]
AID - 10.1194/jlr.M036343 [doi]
PST - ppublish
SO  - J Lipid Res. 2013 Jul;54(7):1877-83. doi: 10.1194/jlr.M036343. Epub 2013 May 10.