PMID- 2366793
OWN - NLM
STAT- MEDLINE
DCOM- 19900813
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 236
IP  - 1
DP  - 1990 Jul
TI  - The induction and repair of DNA damage detected by the DNA precipitation assay in 
      Chinese hamster ovary cells treated with acridine orange + visible light.
PG  - 119-27
AB  - The photodynamic effect of the dye acridine orange (AO) in combination with 
      visible light (400-700 nm) was studied in Chinese hamster ovary (CHO) cells, the 
      endpoints investigated being induction, as well as repair, of DNA strand breaks. 
      Cells were treated for 20 min with AO (0.1-3.0 micrograms/ml), washed free of 
      excess dye and subsequently exposed to low doses of visible light (2 x 40 W/8 
      W/m2) for 5-15 min. AO proved to be an efficient sensitizer for light-induced DNA 
      strand breaks, detected with the DNA precipitation assay, and expressed as 
      percentage of DNA precipitated. The induction of breaks was linear up to 0.5 
      micrograms/ml AO + 10 min of light, which corresponds to 55% precipitated DNA, 
      and was dependent on the concentration of AO as well as on the dose of light 
      delivered. As a comparison, 18 Gy of X-rays was required to yield an equivalent 
      amount of induced DNA strand breaks. The rejoining of the light-induced DNA 
      strand breaks was studied by incubating the AO-sensitized cells for 30-120 min at 
      37 degrees C directly after light exposure. A fast recover of 67-91% of the 
      damage (compared to initial damage, recovery time = 0, and dependent on the 
      concentration of AO) was observed during the first 30 min of incubation. However, 
      a significant amount of DNA damage remained after 2 h of recovery. These 
      remaining, long-lived lesions might be involved in the photoinduced and 
      acridine-sensitized chromosomal aberrations and sister-chromatid exchanges (SCE). 
      The significance of these observations is discussed in relation to AO-sensitized 
      and photoinduced DNA damage and chromosomal alterations.
FAU - Uggla, A H
AU  - Uggla AH
AD  - Department of Genetics, University of Uppsala, Sweden.
FAU - Sundell-Bergman, S
AU  - Sundell-Bergman S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*pharmacology
MH  - Animals
MH  - Cell Line
MH  - Cricetinae
MH  - Cricetulus
MH  - DNA/drug effects/*radiation effects
MH  - *DNA Damage
MH  - *DNA Repair
MH  - Dose-Response Relationship, Radiation
MH  - Female
MH  - *Light
MH  - Ovary
EDAT- 1990/07/01 00:00
MHDA- 1990/07/01 00:01
CRDT- 1990/07/01 00:00
PHST- 1990/07/01 00:00 [pubmed]
PHST- 1990/07/01 00:01 [medline]
PHST- 1990/07/01 00:00 [entrez]
AID - 0921-8777(90)90039-8 [pii]
AID - 10.1016/0921-8777(90)90039-8 [doi]
PST - ppublish
SO  - Mutat Res. 1990 Jul;236(1):119-27. doi: 10.1016/0921-8777(90)90039-8.