PMID- 23669610
OWN - NLM
STAT- MEDLINE
DCOM- 20131024
LR  - 20130528
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 929
DP  - 2013 Jun 15
TI  - Study on urinary profile of inborn errors of metabolism by 18-crown-6 modified 
      capillary electrophoresis with laser-induced fluorescence detection.
PG  - 102-6
LID - S1570-0232(13)00226-2 [pii]
LID - 10.1016/j.jchromb.2013.04.016 [doi]
AB  - Newborn screening in urine is important for the diagnosis of many inborn errors 
      of metabolism (IEM). Capillary electrophoresis with laser-induced fluorescence 
      detection (CE-LIF) is a major technological advance in screening IEM. It has the 
      advantage of sensitive and simultaneous multiple disease screening with minimal 
      sample requirement. The analytes were derivatized with fluorescein isothiocyanate 
      (FITC) prior to CE-LIF analysis. In urine samples, free amino acids (AAs) were 
      well separated from other coexisting components, exhibiting a linear calibration 
      over the concentration range 0.01-5.0mumol/L with the limits of detection (LODs) 
      ranging from 0.005 to 0.010mumol/L. The relative standard deviations (RSDs) were 
      in the range 0.1-1.0% for peak area, and 0.2-1.0% for migration time, 
      respectively. Under optimized conditions, the method presented here has been 
      successfully used for the simultaneous and sensitive analysis of seven AAs in 
      urine samples of newborn babies, and evaluating the effect of therapy as well.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Zhang, Jun-Bo
AU  - Zhang JB
AD  - Department of Chemistry, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China.
FAU - Li, Meng-Jie
AU  - Li MJ
FAU - Li, Zhuo
AU  - Li Z
FAU - Yan, Xiao-Jing
AU  - Yan XJ
FAU - Yuan, Jia-Qun
AU  - Yuan JQ
FAU - Dong, Wen-Xia
AU  - Dong WX
FAU - Zhang, Yu
AU  - Zhang Y
FAU - Chu, Qing-Cui
AU  - Chu QC
FAU - Ye, Jian-Nong
AU  - Ye JN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130418
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Crown Ethers)
RN  - 63J177NC5B (18-crown-6)
SB  - IM
MH  - Crown Ethers/*chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Humans
MH  - Infant, Newborn
MH  - *Lasers
MH  - Metabolism, Inborn Errors/*urine
MH  - Reproducibility of Results
MH  - Spectrometry, Fluorescence/*methods
EDAT- 2013/05/15 06:00
MHDA- 2013/10/25 06:00
CRDT- 2013/05/15 06:00
PHST- 2013/02/05 00:00 [received]
PHST- 2013/04/08 00:00 [revised]
PHST- 2013/04/12 00:00 [accepted]
PHST- 2013/05/15 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2013/10/25 06:00 [medline]
AID - S1570-0232(13)00226-2 [pii]
AID - 10.1016/j.jchromb.2013.04.016 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jun 15;929:102-6. doi: 
      10.1016/j.jchromb.2013.04.016. Epub 2013 Apr 18.