PMID- 23671272
OWN - NLM
STAT- MEDLINE
DCOM- 20131216
LR  - 20130802
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 27
IP  - 8
DP  - 2013 Aug
TI  - A novel peptide carrier for efficient targeting of antigens and nucleic acids to 
      dendritic cells.
PG  - 3272-83
LID - 10.1096/fj.12-224758 [doi]
AB  - Dendritic cells (DCs) initiate host immune responses by presenting captured 
      antigens to naive T cells. Hence, DC-binding peptides may be used for antigen 
      targeting to boost naive and memory immune responses. By biopanning peptide phage 
      libraries on human monocyte-derived DCs, we identified novel DC-binding peptides. 
      One of the selected phages, displaying the NW peptide (NWYLPWLGTNDW), bound DCs 
      with high affinity, and its binding was inhibited by the corresponding synthetic 
      peptide. Antigenic peptides or proteins conjugated to the NW peptide bound to DCs 
      and were internalized without negative effects on DC phenotype and function. Ex 
      vivo targeted delivery of CMV-pp65 peptides to DCs via the NW peptide increased 
      T-cell responses in HLA-A2(+)/CMV(+) donors compared to untargeted peptides 
      (P<0.001). Stimulation of CD45RO-depleted peripheral blood mononuclear cells from 
      CMV(-) donors with the NW-pp65 fusion peptides expanded pp65-specific precursor T 
      cells. Moreover, the NW peptide mediated small interfering RNA delivery to DCs, 
      and a significant gene silencing was obtained. Collectively, the data reveal that 
      proteins and nucleic acids can be directed to DCs through the NW peptide, 
      enabling effective uptake and functional effects such as T-cell activation in the 
      context of MHC class I and II molecules.
FAU - Sioud, Mouldy
AU  - Sioud M
AD  - Department of Immunology, Oslo University, Radium Hospital, Montebello, N-0310 
      Oslo, Norway. mosioud@medisin.uio.no
FAU - Skorstad, Gjertrud
AU  - Skorstad G
FAU - Mobergslien, Anne
AU  - Mobergslien A
FAU - Saeboe-Larssen, Stein
AU  - Saeboe-Larssen S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130513
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Antigens)
RN  - 0 (Carrier Proteins)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Nucleic Acids)
RN  - 0 (Oligopeptides)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (cytomegalovirus matrix protein 65kDa)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigen Presentation/immunology
MH  - Antigens/*immunology/metabolism
MH  - Binding, Competitive/immunology
MH  - Carrier Proteins/immunology/metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/metabolism
MH  - Flow Cytometry
MH  - HLA-A2 Antigen/immunology/metabolism
MH  - Humans
MH  - Leukocytes, Mononuclear/immunology/metabolism
MH  - Lymphocyte Activation/immunology
MH  - Microscopy, Fluorescence
MH  - Monocytes/immunology/metabolism
MH  - Nucleic Acids/*immunology/metabolism
MH  - Oligopeptides/*immunology/metabolism
MH  - Phosphoproteins/immunology/metabolism
MH  - Protein Binding/immunology
MH  - RNA, Small Interfering/genetics/immunology/metabolism
MH  - T-Lymphocytes/immunology/metabolism
MH  - Viral Matrix Proteins/immunology/metabolism
OTO - NOTNLM
OT  - cancer vaccines
OT  - cross presentation
OT  - phage display
OT  - siRNA
EDAT- 2013/05/15 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/05/15 06:00
PHST- 2013/05/15 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
AID - fj.12-224758 [pii]
AID - 10.1096/fj.12-224758 [doi]
PST - ppublish
SO  - FASEB J. 2013 Aug;27(8):3272-83. doi: 10.1096/fj.12-224758. Epub 2013 May 13.