PMID- 23671824
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130515
LR  - 20211021
IS  - 2228-5806 (Print)
IS  - 2228-5814 (Electronic)
IS  - 2228-5806 (Linking)
VI  - 13
IP  - 1
DP  - 2011 Spring
TI  - Neuroprotective properties of melissa officinalis L. Extract against 
      ecstasy-induced neurotoxicity.
PG  - 25-30
AB  - OBJECTIVE: The aim of the present study was to investigate the neuroprotective 
      effects of Melissa officinalis, a major antioxidant plant, against neuron 
      toxicity in hippocampal primary culture induced by 
      3,4-methylenedioxymethamphetamine (MDMA) or ecstasy, one of the most abused 
      drugs, which causes neurotoxicity. MATERIALS AND METHODS: 3-(4,5-dimethyl-2 
      thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) assay was used to assess 
      mitochondrial activity, reflecting cell survival. Caspase-3 activity assay and 
      Hoechst / propiedium iodide (PI) staining were done to show apoptotic cell death. 
      RESULTS: A high dose of ecstasy caused profound mitochondrial dysfunction, around 
      40% less than the control value, and increased apoptotic neuronal death to around 
      35% more than the control value in hippocampal neuronal culture. Co-treatment 
      with Melissa officinalis significantly reversed these damages to around 15% and 
      20% respectively of the MDMA alone group, and provided protection against 
      MDMA-induced mitochondrial dysfunction and apoptosis in neurons. CONCLUSION: 
      Melissa officinalis has revealed neuroprotective effects against apoptosis 
      induced by MDMA in the primary neurons of hippocampal culture, which could be due 
      to its free radical scavenging properties and monoamine oxidase (MAO) inhibitory 
      effects.
FAU - Hassanzadeh, Gholamreza
AU  - Hassanzadeh G
AD  - 1. Anatomy Department, School of Medicine, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Pasbakhsh, Parichehr
AU  - Pasbakhsh P
FAU - Akbari, Mohammad
AU  - Akbari M
FAU - Shokri, Saeed
AU  - Shokri S
FAU - Ghahremani, Mohammadhosein
AU  - Ghahremani M
FAU - Amin, Gholamreza
AU  - Amin G
FAU - Kashani, Iraj
AU  - Kashani I
FAU - Azami Tameh, Abolfazl
AU  - Azami Tameh A
LA  - eng
PT  - Journal Article
DEP - 20110421
PL  - Iran
TA  - Cell J
JT  - Cell journal
JID - 101566618
PMC - PMC3652537
OTO - NOTNLM
OT  - Ecstasy
OT  - MDMA
OT  - Melissa Officinalis
OT  - Neurotoxicity
EDAT- 2011/04/01 00:00
MHDA- 2011/04/01 00:01
PMCR- 2011/04/01
CRDT- 2013/05/15 06:00
PHST- 2010/04/04 00:00 [received]
PHST- 2010/08/10 00:00 [accepted]
PHST- 2013/05/15 06:00 [entrez]
PHST- 2011/04/01 00:00 [pubmed]
PHST- 2011/04/01 00:01 [medline]
PHST- 2011/04/01 00:00 [pmc-release]
PST - ppublish
SO  - Cell J. 2011 Spring;13(1):25-30. Epub 2011 Apr 21.