PMID- 23671840
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130515
LR  - 20211021
IS  - 2314-436X (Print)
IS  - 2314-4378 (Electronic)
IS  - 2314-436X (Linking)
VI  - 2013
DP  - 2013
TI  - Characterization of the OmyY1 Region on the Rainbow Trout Y Chromosome.
PG  - 261730
LID - 10.1155/2013/261730 [doi]
LID - 261730
AB  - We characterized the male-specific region on the Y chromosome of rainbow trout, 
      which contains both sdY (the sex-determining gene) and the male-specific genetic 
      marker, OmyY1. Several clones containing the OmyY1 marker were screened from a 
      BAC library from a YY clonal line and found to be part of an 800 kb BAC contig. 
      Using fluorescence in situ hybridization (FISH), these clones were localized to 
      the end of the short arm of the Y chromosome in rainbow trout, with an additional 
      signal on the end of the X chromosome in many cells. We sequenced a minimum 
      tiling path of these clones using Illumina and 454 pyrosequencing. The region is 
      rich in transposons and rDNA, but also appears to contain several single-copy 
      protein-coding genes. Most of these genes are also found on the X chromosome; and 
      in several cases sex-specific SNPs in these genes were identified between the 
      male (YY) and female (XX) homozygous clonal lines. Additional genes were 
      identified by hybridization of the BACs to the cGRASP salmonid 4x44K oligo 
      microarray. By BLASTn evaluations using hypothetical transcripts of OmyY1-linked 
      candidate genes as query against several EST databases, we conclude at least 12 
      of these candidate genes are likely functional, and expressed.
FAU - Phillips, Ruth B
AU  - Phillips RB
AD  - School of Biological Sciences, Washington State University Vancouver, 14204 NE, 
      Salmon Creek Avenue, Vancouver, WA 98686-9600, USA ; Center for Reproductive 
      Biology, Washington State University, Pullman, WA 99164-7520, USA.
FAU - Dekoning, Jenefer J
AU  - Dekoning JJ
FAU - Brunelli, Joseph P
AU  - Brunelli JP
FAU - Faber-Hammond, Joshua J
AU  - Faber-Hammond JJ
FAU - Hansen, John D
AU  - Hansen JD
FAU - Christensen, Kris A
AU  - Christensen KA
FAU - Renn, Suzy C P
AU  - Renn SC
FAU - Thorgaard, Gary H
AU  - Thorgaard GH
LA  - eng
PT  - Journal Article
DEP - 20130311
PL  - United States
TA  - Int J Genomics
JT  - International journal of genomics
JID - 101605206
PMC - PMC3647546
EDAT- 2013/05/15 06:00
MHDA- 2013/05/15 06:01
PMCR- 2013/03/11
CRDT- 2013/05/15 06:00
PHST- 2012/09/18 00:00 [received]
PHST- 2012/12/26 00:00 [revised]
PHST- 2013/01/17 00:00 [accepted]
PHST- 2013/05/15 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2013/05/15 06:01 [medline]
PHST- 2013/03/11 00:00 [pmc-release]
AID - 10.1155/2013/261730 [doi]
PST - ppublish
SO  - Int J Genomics. 2013;2013:261730. doi: 10.1155/2013/261730. Epub 2013 Mar 11.