PMID- 23671888
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130515
LR  - 20211021
IS  - 2314-6745 (Print)
IS  - 2314-6753 (Electronic)
VI  - 2013
DP  - 2013
TI  - Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus.
PG  - 721493
LID - 10.1155/2013/721493 [doi]
LID - 721493
AB  - Objectives. To study the correlation between the methylation of protein kinase C 
      epsilon zeta (PRKCZ) gene promoters and type 2 diabetes mellitus (T2DM). Methods. 
      The case-control method was implemented in 272 unrelated to one another cases in 
      Shiyan People's Hospital. Of those, 152 were diagnosed as T2DM cases, and the 
      other 120 cases were healthy individuals visiting the hospital for a physical 
      examination. The subjects were first divided into two groups: the T2DM group and 
      the normal control (NC) group. Next, methylated DNA immunoprecipitation chip 
      (MeDIP-chip) was used for detection. Bisulfite sequencing PCR (BSP) and gene 
      sequencing were then performed to detect and analyze the correlation between 
      PRKCZ gene promoter methylation and T2DM. Finally, Western blotting was applied 
      to determine the serum level of PRKCZ. The data were then analyzed with the 
      statistics analyzing software SPSS 17.0. Results. In contrast with cases in NC, 
      T2DM patients showed a high level of methylation, with 7 of 9 CpG sites were 
      shown to be methylated, whereas, in the control group, only one CpG site was 
      found to be methylated. The methylated CpG sites for the two groups showed marked 
      differences (P < 0.01). Additionally, the level of PRKCZ was decreased in T2DM 
      subjects, and the difference between the two groups was statistically significant 
      (P < 0.05). Discussion. This study suggests that the PRKCZ gene is the 
      hypermethylated gene of T2DM and the hypermethylation PRKCZ gene may be involved 
      in the pathogenesis of T2DM.
FAU - Zou, Li
AU  - Zou L
AD  - Clinical Laboratory of Renmin Hospital, The Third Affiliated Hospital of Hubei 
      University of Medicine, 39 Chaoyang Road, Shiyan, Hubei Province 442000, China.
FAU - Yan, Shirong
AU  - Yan S
FAU - Guan, Xueping
AU  - Guan X
FAU - Pan, Yunjun
AU  - Pan Y
FAU - Qu, Xin
AU  - Qu X
LA  - eng
PT  - Journal Article
DEP - 20130316
PL  - England
TA  - J Diabetes Res
JT  - Journal of diabetes research
JID - 101605237
PMC - PMC3647574
EDAT- 2013/05/15 06:00
MHDA- 2013/05/15 06:01
PMCR- 2013/03/16
CRDT- 2013/05/15 06:00
PHST- 2012/11/07 00:00 [received]
PHST- 2013/02/21 00:00 [accepted]
PHST- 2013/05/15 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2013/05/15 06:01 [medline]
PHST- 2013/03/16 00:00 [pmc-release]
AID - 10.1155/2013/721493 [doi]
PST - ppublish
SO  - J Diabetes Res. 2013;2013:721493. doi: 10.1155/2013/721493. Epub 2013 Mar 16.