PMID- 23671910
OWN - NLM
STAT- MEDLINE
DCOM- 20131227
LR  - 20161125
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 138
IP  - 12
DP  - 2013 Jun 21
TI  - A tyrosinase biosensor based on ordered mesoporous carbon-Au/L-lysine/Au 
      nanoparticles for simultaneous determination of hydroquinone and catechol.
PG  - 3552-60
LID - 10.1039/c3an36928e [doi]
AB  - A novel biosensor was developed based on tyrosinase immobilization with ordered 
      mesoporous carbon-Au (OMC-Au), L-lysine membrane and Au nanoparticles on a glassy 
      carbon electrode (GCE). It was applied for the simultaneous determination of 
      dihydroxybenzene isomers using differential pulse voltammetry (DPV). The 
      tyrosinase/OMC-Au/L-lysine/Au film was characterized by scanning electron 
      microscopy (SEM) and impedance spectra. Under optimized conditions, the DPV study 
      results for two isomers, hydroquinone (HQ, 1,4-dihydroxybenzene) and catechol 
      (CC, 1,2-dihydroxybenzene) showed low peak potentials, and the peak-to-peak 
      difference was about 135.85 mV, which ensured the anti-interference ability of 
      the biosensor and made simultaneous detection of dihydroxybenzene isomers 
      possible in real samples. DPV peak currents increased linearly with concentration 
      over the range of 4.0 x 10(-7) to 8.0 x 10(-5) M, and the detection limits of 
      hydroquinone and catechol were 5 x 10(-8) M and 2.5 x 10(-8) M (S/N = 3), 
      respectively. The tyrosinase biosensor exhibited good repeatability and 
      stability. In addition, the response mechanism of enzyme catalysed redox on the 
      OMC-Au/L-lysine/Au film modified electrode based on electrochemical study was 
      discussed. The proposed method could be extended for the development of other 
      enzyme-based biosensors.
FAU - Tang, Lin
AU  - Tang L
AD  - College of Environmental Science and Engineering, Hunan University, Changsha, 
      410082, PR China. tanglin@hnu.edu.cn
FAU - Zhou, Yaoyu
AU  - Zhou Y
FAU - Zeng, Guangming
AU  - Zeng G
FAU - Li, Zhen
AU  - Li Z
FAU - Liu, Yuanyuan
AU  - Liu Y
FAU - Zhang, Yi
AU  - Zhang Y
FAU - Chen, Guiqiu
AU  - Chen G
FAU - Yang, Guide
AU  - Yang G
FAU - Lei, Xiaoxia
AU  - Lei X
FAU - Wu, Mengshi
AU  - Wu M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130513
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Catechols)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Hydroquinones)
RN  - 0 (Membranes, Artificial)
RN  - 059QF0KO0R (Water)
RN  - 7440-44-0 (Carbon)
RN  - 7440-57-5 (Gold)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
RN  - K3Z4F929H6 (Lysine)
RN  - LF3AJ089DQ (catechol)
RN  - XV74C1N1AE (hydroquinone)
SB  - IM
MH  - Agaricales/enzymology
MH  - Biocatalysis
MH  - Biosensing Techniques/*methods
MH  - Carbon/*chemistry
MH  - Catechols/*analysis/chemistry
MH  - Electrochemistry
MH  - Electrodes
MH  - Enzymes, Immobilized/chemistry/metabolism
MH  - Gold/*chemistry
MH  - Hydroquinones/*analysis/chemistry
MH  - Isomerism
MH  - Kinetics
MH  - Lysine/*chemistry
MH  - Membranes, Artificial
MH  - Metal Nanoparticles/chemistry
MH  - Monophenol Monooxygenase/chemistry/*metabolism
MH  - Oxidation-Reduction
MH  - Porosity
MH  - Time Factors
MH  - Water/chemistry
EDAT- 2013/05/15 06:00
MHDA- 2013/12/29 06:00
CRDT- 2013/05/15 06:00
PHST- 2013/05/15 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2013/12/29 06:00 [medline]
AID - 10.1039/c3an36928e [doi]
PST - ppublish
SO  - Analyst. 2013 Jun 21;138(12):3552-60. doi: 10.1039/c3an36928e. Epub 2013 May 13.