PMID- 23672052 OWN - NLM STAT- MEDLINE DCOM- 20140619 LR - 20161125 IS - 1001-5302 (Print) IS - 1001-5302 (Linking) VI - 38 IP - 2 DP - 2013 Jan TI - [Experimental studies on pharmacokinetics of three components in Buyanghuanwu injection on base of total quantum statistical moment]. PG - 253-62 AB - OBJECTIVE: To verify established the total quantum statistic moments model with astragaloside IV, paeoniflorin, tetramethylpyrazine in Buyanghuanwu injection, in order to establish a pharmacokinetic experimental method with multi-component traditional Chinese medicine (TCM) compound system. METHOD: The RP-HPLC was adopted, with the chromatographic column of C18, 4.6 mm x 250 mm, 5 microm. As for astragaloside IV, the ELSD detector was adopted with acetonitrile-water (35: 65) as the mobile phase at 1 mL x min(-1); the pressure of column was (15.0 +/- 0.408) MPa, the column temperature was 30 degrees C. Regarding paeoniflorin and tetramethylpyrazine, the detection of wavelengths was 254 nm, with acetonitrile-water (35:65) as the mobile phase at 1 mL x min(-1), the column pressure of (15.17 +/- 0.41) MPa. The pharmacokinetic parameters for single component were dealt with DAS and the total quantum statistical moment (TQSM) parameters were calculated using formulations. RESULT: All of the three components followed the two compartmental pharkacokinetic model (P < 0.01) in rats. Compared with the superimposed total concentration, each single component showed difference in parameters up to 10 000 times at most, whereas the RSD of TQSM parameters was 3.510%. The TQSM pharmacokinetic parameters of the three components in Buyanghuanwu injection showed that AUC(t), MRT(t), VRT(t), CL(t), V(t), were (119.8 +/- 27.20) g x min x L(-1), (210.0 +/- 54.49) min, (5.608 +/- 2.723) x 10(4) min2, (0.319 6 +/- 0.068 8) mL x min(-1) x kg(-1) and (64.12 +/- 8.243) mL x kg(-1), respectively, suggesting that the half-life time for the three components were (145.5 +/- 37.76) min and 95% of them were metabolized within 0-674. 2 min. CONCLUSION: The TQSM can be used to study pharmacokinetic parameters of multi-component TCM compound, because the method can characterize the pharmacokinetic regularity of quantum-time change in a multi-component system. FAU - He, Fu-Yuan AU - He FY AD - Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. pharmsharking@tom.com FAU - Deng, Kai-Wen AU - Deng KW FAU - Liu, Wen-Long AU - Liu WL FAU - Shi, Ji-Lian AU - Shi JL FAU - Wu, Yong AU - Wu Y FAU - Liu, Wei AU - Liu W FAU - He, Qing-Ping AU - He QP FAU - Li, Bo AU - Li B LA - chi PT - Journal Article PL - China TA - Zhongguo Zhong Yao Za Zhi JT - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JID - 8913656 RN - 0 (Benzoates) RN - 0 (Bridged-Ring Compounds) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Glucosides) RN - 0 (Monoterpenes) RN - 0 (Pyrazines) RN - 0 (Saponins) RN - 0 (Triterpenes) RN - 0 (buyang huanwu) RN - 21AIQ4EV64 (peoniflorin) RN - 3A592W8XKE (astragaloside A) RN - V80F4IA5XG (tetramethylpyrazine) SB - IM MH - Animals MH - Benzoates/*pharmacokinetics MH - Bridged-Ring Compounds/*pharmacokinetics MH - Chromatography, High Pressure Liquid MH - Drugs, Chinese Herbal/*pharmacokinetics MH - Glucosides/*pharmacokinetics MH - Male MH - Medicine, Chinese Traditional MH - *Models, Statistical MH - Monoterpenes MH - Pyrazines/*pharmacokinetics MH - Rats MH - Saponins/*pharmacokinetics MH - Triterpenes/*pharmacokinetics EDAT- 2013/05/16 06:00 MHDA- 2014/06/20 06:00 CRDT- 2013/05/16 06:00 PHST- 2013/05/16 06:00 [entrez] PHST- 2013/05/16 06:00 [pubmed] PHST- 2014/06/20 06:00 [medline] PST - ppublish SO - Zhongguo Zhong Yao Za Zhi. 2013 Jan;38(2):253-62.