PMID- 23672816
OWN - NLM
STAT- MEDLINE
DCOM- 20131024
LR  - 20131121
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 929
DP  - 2013 Jun 15
TI  - Simultaneous quantification of amphetamine, opiates, ketamine and relative 
      metabolites in urine for confirmatory analysis by liquid chromatography tandem 
      mass spectrometry.
PG  - 133-41
LID - S1570-0232(13)00180-3 [pii]
LID - 10.1016/j.jchromb.2013.03.022 [doi]
AB  - The rise in amphetamine, ketamine and opiates abuse in Taiwan has created a need 
      for a reliable confirmatory assay. A method that combines superficially porous 
      liquid chromatography tandem mass spectrometry (LC-MS/MS) with solid-phase 
      extraction (SPE) was developed for the simultaneous quantification of 
      amphetamine, 3,4-methylenedioxymethamphetamine (MDMA), ketamine, opiates, and 
      their corresponding metabolites in urine. The total run time of the method was 
      6.7min including equilibration time. The method was validated in accordance with 
      the European Commission (EC) Decision 2002/642/EC. The within- and between-day 
      precision was below 13.6% and the accuracy ranged from -17.1% to +9.9% for all 
      analytes. Ion suppression was observed but compensated by using deuterated 
      internal standards. No carryover was detected and the analytes were stable at 
      room temperature for 16h, and for 72h at 4 degrees C, and three-thaw cycles. The method 
      was further validated by comparison with a reference gas chromatography-mass 
      spectrometry (GC-MS) method, using 52 authentic urine samples. The results 
      indicated that for the target analytes studied, the LC-MS/MS analysis was as 
      precise, accurate, and specific as the GC-MS method. In conclusion, the present 
      LC-MS/MS method is robust and reliable, and suitable for use as a confirmation 
      assay in the simultaneous urine drug testing and quantification of amphetamines, 
      ketamines, and opiates.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Lin, Huei-Ru
AU  - Lin HR
AD  - Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, 
      Taiwan. rita0107@mail.tcu.edu.tw
FAU - Choi, Ka-Ian
AU  - Choi KI
FAU - Lin, Tzu-Chieh
AU  - Lin TC
FAU - Hu, Anren
AU  - Hu A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130418
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Amphetamines)
RN  - 0 (Opioid Peptides)
RN  - 690G0D6V8H (Ketamine)
SB  - IM
MH  - Amphetamines/*urine
MH  - Chromatography, Liquid/*methods
MH  - Humans
MH  - Ketamine/*urine
MH  - Opioid Peptides/*urine
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2013/05/16 06:00
MHDA- 2013/10/25 06:00
CRDT- 2013/05/16 06:00
PHST- 2012/11/13 00:00 [received]
PHST- 2013/03/12 00:00 [revised]
PHST- 2013/03/24 00:00 [accepted]
PHST- 2013/05/16 06:00 [entrez]
PHST- 2013/05/16 06:00 [pubmed]
PHST- 2013/10/25 06:00 [medline]
AID - S1570-0232(13)00180-3 [pii]
AID - 10.1016/j.jchromb.2013.03.022 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jun 15;929:133-41. doi: 
      10.1016/j.jchromb.2013.03.022. Epub 2013 Apr 18.