PMID- 23674607
OWN - NLM
STAT- MEDLINE
DCOM- 20140515
LR  - 20211021
IS  - 1349-9157 (Electronic)
IS  - 0449-3060 (Print)
IS  - 0449-3060 (Linking)
VI  - 54
IP  - 6
DP  - 2013 Nov 1
TI  - Comparison of the repair of potentially lethal damage after low- and high-LET 
      radiation exposure, assessed from the kinetics and fidelity of chromosome 
      rejoining in normal human fibroblasts.
PG  - 989-97
LID - 10.1093/jrr/rrt031 [doi]
AB  - Potentially lethal damage (PLD) and its repair (PLDR) were studied in confluent 
      human fibroblasts by analyzing the kinetics of chromosome break rejoining after 
      X-ray or heavy-ion exposures. Cells were either held in the non-cycling G0 phase 
      of the cell cycle for 12 h, or forced to proliferate immediately after 
      irradiation. Fusion premature chromosome condensation (PCC) was combined with 
      fluorescence in situ hybridization (FISH) to study chromosomal aberrations in 
      interphase. The culture condition had no impact on the rejoining kinetics of PCC 
      breaks during the 12 h after X-ray or heavy-ion irradiation. However, 12 h after 
      X-ray and silicon irradiation, cycling cells had more chromosome exchanges than 
      non-cycling cells. After 6 Gy X-rays, the yield of exchanges in cycling cells was 
      2.8 times higher than that in non-cycling cells, and after 2 Gy of 55 keV/mum 
      silicon ions the yield of exchanges in cycling cells was twice that of 
      non-cycling cells. In contrast, after exposure to 2 Gy 200-keV/mum or 440-keV/mum 
      iron ions the yield of exchanges was similar in non-cycling and cycling cells. 
      Since the majority of repair in G0/G1 occurs via the non-homologous end joining 
      process (NHEJ), increased PLDR in X-ray and silicon-ion irradiated cells may 
      result from improved cell cycle-specific rejoining fidelity through the NHEJ 
      pathway, which is not the case in high-LET iron-ion irradiated cells.
FAU - Liu, Cuihua
AU  - Liu C
AD  - Research Center for Charged Particle Therapy, National Institute of Radiological 
      Sciences, Chiba 263-8555, Japan.
FAU - Kawata, Tetsuya
AU  - Kawata T
FAU - Zhou, Guangming
AU  - Zhou G
FAU - Furusawa, Yoshiya
AU  - Furusawa Y
FAU - Kota, Ryuichi
AU  - Kota R
FAU - Kumabe, Atsuhiro
AU  - Kumabe A
FAU - Sutani, Shinya
AU  - Sutani S
FAU - Fukada, Junichi
AU  - Fukada J
FAU - Mishima, Masayo
AU  - Mishima M
FAU - Shigematsu, Naoyuki
AU  - Shigematsu N
FAU - George, Kerry
AU  - George K
FAU - Cucinotta, Francis
AU  - Cucinotta F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130514
PL  - England
TA  - J Radiat Res
JT  - Journal of radiation research
JID - 0376611
SB  - IM
MH  - Apoptosis/*genetics/radiation effects
MH  - Cell Cycle/*physiology/radiation effects
MH  - Cells, Cultured
MH  - Chromosome Aberrations/*radiation effects
MH  - DNA End-Joining Repair/*genetics/*radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Fibroblasts/*cytology/*physiology/radiation effects
MH  - Humans
MH  - Kinetics
MH  - Linear Energy Transfer/genetics/*radiation effects
MH  - Radiation Dosage
PMC - PMC3823769
OTO - NOTNLM
OT  - FISH (fluorescence in situ hybridization)
OT  - PLDR (potentially lethal damage repair)
OT  - heavy ion
OT  - misrepair
OT  - premature chromosome condensation
EDAT- 2013/05/16 06:00
MHDA- 2014/05/16 06:00
PMCR- 2013/05/14
CRDT- 2013/05/16 06:00
PHST- 2013/05/16 06:00 [entrez]
PHST- 2013/05/16 06:00 [pubmed]
PHST- 2014/05/16 06:00 [medline]
PHST- 2013/05/14 00:00 [pmc-release]
AID - rrt031 [pii]
AID - 10.1093/jrr/rrt031 [doi]
PST - ppublish
SO  - J Radiat Res. 2013 Nov 1;54(6):989-97. doi: 10.1093/jrr/rrt031. Epub 2013 May 14.