PMID- 23675932
OWN - NLM
STAT- MEDLINE
DCOM- 20140218
LR  - 20130610
IS  - 1365-2842 (Electronic)
IS  - 0305-182X (Linking)
VI  - 40
IP  - 7
DP  - 2013 Jul
TI  - Experimentally created unilateral anterior crossbite induces a degenerative 
      ossification phenotype in mandibular condyle of growing Sprague-Dawley rats.
PG  - 500-8
LID - 10.1111/joor.12072 [doi]
AB  - The effect of unilateral anterior crossbite on the remodelling of mandibular 
      condyle needs to be investigated. This study aimed to investigate the effects of 
      experimentally created unilateral anterior crossbite on the remodelling of 
      mandibular condyle and explore the changes in the expression of relevant 
      transcription factors and growth factors. The experimental unilateral anterior 
      crossbite was created in 6-week-old female growing rats by bonding metal tubes to 
      the left pairs of incisors. Remodelings of mandibular condylar cartilage was 
      assessed histologically at 2, 4 and 8 weeks. Protein and mRNA levels of Sox9, 
      runt-related transcription factor 2 (Runx2), Osterix (Osx), transforming growth 
      factor beta 1 (TGFbeta1), transforming growth factor beta receptor 2 (TGFbetar2) and 
      type X collagen (ColX) were investigated by immunohistochemistry and real-time 
      PCR, while alkaline phosphatise (ALP) by histochemistry and real-time PCR. 
      Decreased ratio of hypertrophic cartilage layer was noticed in the 4w 
      experimental group versus controls. At all the time points, the expression of 
      Sox9 and ALP increased but that of TGFbeta1 and TGFbetar2 decreased in experimental 
      groups (P < 0.05). The expression of Runx2, Osx and Col X increased at 2w, but 
      decrease at 4w (P < 0.05). The results that obvious cartilage degradation and 
      altered expression of related transcription factors and growth factors were 
      detected in the mandibular condyles of the experimental group suggested that the 
      present unilateral anterior crossbite plays an adverse role in the TMJ, and thus 
      leading to the degenerative endochondral ossification.
CI  - (c) 2013 John Wiley & Sons Ltd.
FAU - Zhang, X
AU  - Zhang X
AD  - Department of Oral Anatomy and Physiology and TMD, School of Stomatology, Fourth 
      Military Medical University, Xi'an, China.
FAU - Dai, J
AU  - Dai J
FAU - Lu, L
AU  - Lu L
FAU - Zhang, J
AU  - Zhang J
FAU - Zhang, M
AU  - Zhang M
FAU - Wang, Y
AU  - Wang Y
FAU - Guo, M
AU  - Guo M
FAU - Wang, X
AU  - Wang X
FAU - Wang, M
AU  - Wang M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130515
PL  - England
TA  - J Oral Rehabil
JT  - Journal of oral rehabilitation
JID - 0433604
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/metabolism/pathology
MH  - Chondrocytes/metabolism
MH  - Female
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Malocclusion/*physiopathology
MH  - Mandibular Condyle/*growth & development/metabolism/pathology
MH  - Ossification, Heterotopic/*genetics
MH  - Phenotype
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Transcription Factors/metabolism
EDAT- 2013/05/17 06:00
MHDA- 2014/02/19 06:00
CRDT- 2013/05/17 06:00
PHST- 2013/04/26 00:00 [accepted]
PHST- 2013/05/17 06:00 [entrez]
PHST- 2013/05/17 06:00 [pubmed]
PHST- 2014/02/19 06:00 [medline]
AID - 10.1111/joor.12072 [doi]
PST - ppublish
SO  - J Oral Rehabil. 2013 Jul;40(7):500-8. doi: 10.1111/joor.12072. Epub 2013 May 15.