PMID- 23676272
OWN - NLM
STAT- MEDLINE
DCOM- 20130806
LR  - 20211021
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 110
IP  - 23
DP  - 2013 Jun 4
TI  - Arid5a controls IL-6 mRNA stability, which contributes to elevation of IL-6 level 
      in vivo.
PG  - 9409-14
LID - 10.1073/pnas.1307419110 [doi]
AB  - Posttranscriptional regulation of IL-6 has been largely uncharacterized, with the 
      exception of the ribonuclease Regnase-1, which prevents autoimmunity by 
      destabilizing IL-6 mRNA. Here, we identified AT-rich interactive 
      domain-containing protein 5A (Arid5a) as a unique RNA binding protein, which 
      stabilizes IL-6 but not TNF-alpha mRNA through binding to the 3' untranslated region 
      of IL-6 mRNA. Arid5a was enhanced in macrophages in response to LPS, IL-1beta, and 
      IL-6. Arid5a deficiency inhibited elevation of IL-6 serum level in LPS-treated 
      mice and suppressed IL-6 levels and the development of T(H)17 cells in 
      experimental autoimmune encephalomyelitis. Importantly, Arid5a inhibited the 
      destabilizing effect of Regnase-1 on IL-6 mRNA. These results indicate that 
      Arid5a plays an important role in promotion of inflammatory processes and 
      autoimmune diseases.
FAU - Masuda, Kazuya
AU  - Masuda K
AD  - Laboratory of Immune Regulation, World Premier International Immunology Frontier 
      Research Center, Osaka University, Osaka 565-0871, Japan.
FAU - Ripley, Barry
AU  - Ripley B
FAU - Nishimura, Riko
AU  - Nishimura R
FAU - Mino, Takashi
AU  - Mino T
FAU - Takeuchi, Osamu
AU  - Takeuchi O
FAU - Shioi, Go
AU  - Shioi G
FAU - Kiyonari, Hiroshi
AU  - Kiyonari H
FAU - Kishimoto, Tadamitsu
AU  - Kishimoto T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130515
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Arid5a protein, mouse)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (interleukin-6, mouse)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 3.1.- (Ribonucleases)
RN  - EC 3.1.- (Zc3h12a protein, mouse)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Animals
MH  - Cell Culture Techniques
MH  - DNA-Binding Proteins/*immunology/metabolism
MH  - Encephalomyelitis, Autoimmune, Experimental/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Interleukin-6/blood/*immunology
MH  - Lipopolysaccharides
MH  - Luciferases
MH  - Macrophages/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA Stability/*immunology
MH  - RNA-Binding Proteins/*immunology/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribonucleases/antagonists & inhibitors
MH  - Transcription Factors/*immunology/metabolism
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC3677444
OTO - NOTNLM
OT  - RNA-protein complex
OT  - immune regulation
COIS- The authors declare no conflict of interest.
EDAT- 2013/05/17 06:00
MHDA- 2013/08/07 06:00
PMCR- 2013/12/04
CRDT- 2013/05/17 06:00
PHST- 2013/05/17 06:00 [entrez]
PHST- 2013/05/17 06:00 [pubmed]
PHST- 2013/08/07 06:00 [medline]
PHST- 2013/12/04 00:00 [pmc-release]
AID - 1307419110 [pii]
AID - 201307419 [pii]
AID - 10.1073/pnas.1307419110 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2013 Jun 4;110(23):9409-14. doi: 
      10.1073/pnas.1307419110. Epub 2013 May 15.