PMID- 23682635
OWN - NLM
STAT- MEDLINE
DCOM- 20140425
LR  - 20211203
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 44
IP  - 1
DP  - 2013 May 17
TI  - Salmonella enterica Typhimurium infection causes metabolic changes in chicken 
      muscle involving AMPK, fatty acid and insulin/mTOR signaling.
PG  - 35
LID - 10.1186/1297-9716-44-35 [doi]
AB  - Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) infection of 
      chickens that are more than a few days old results in asymptomatic cecal 
      colonization with persistent shedding of bacteria. We hypothesized that while the 
      bacterium colonizes and persists locally in the cecum it has systemic effects, 
      including changes to metabolic pathways of skeletal muscle, influencing the 
      physiology of the avian host. Using species-specific peptide arrays to perform 
      kinome analysis on metabolic signaling pathways in skeletal muscle of Salmonella 
      Typhimurium infected chickens, we have observed key metabolic changes that 
      affected fatty acid and glucose metabolism through the 5'-adenosine 
      monophosphate-activated protein kinase (AMPK) and the insulin/mammalian target of 
      rapamycin (mTOR) signaling pathway. Over a three week time course of infection, 
      we observed changes in the phosphorylation state of the AMPK protein, and 
      proteins up and down the pathway. In addition, changes to a large subset of the 
      protein intermediates of the insulin/mTOR pathway in the skeletal muscle were 
      altered by infection. These changes occur in pathways with direct effects on 
      fatty acid and glucose metabolism. This is the first report of significant 
      cellular metabolic changes occurring systemically in chicken due to a Salmonella 
      infection. These results have implications not only for animal production and 
      health but also for the understanding of how Salmonella infection in the 
      intestine can have widespread, systemic effects on the metabolism of chickens 
      without disease-like symptoms.
FAU - Arsenault, Ryan J
AU  - Arsenault RJ
AD  - United States Department of Agriculture, Agricultural Research Service, SPARC, 
      College Station, TX, 77845, USA. ryan.arsenault@usask.ca.
FAU - Napper, Scott
AU  - Napper S
FAU - Kogut, Michael H
AU  - Kogut MH
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130517
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Fatty Acids)
RN  - 0 (Insulin)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Animals
MH  - Antibodies, Bacterial/*metabolism
MH  - *Chickens
MH  - Fatty Acids/metabolism
MH  - Insulin/metabolism
MH  - Muscle, Skeletal/*metabolism/microbiology
MH  - Phosphorylation
MH  - Poultry Diseases/*microbiology
MH  - Protein Array Analysis/veterinary
MH  - Salmonella Infections, Animal/*microbiology
MH  - Salmonella typhimurium/immunology/*physiology
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/metabolism
PMC - PMC3663815
EDAT- 2013/05/21 06:00
MHDA- 2014/04/26 06:00
PMCR- 2013/01/01
CRDT- 2013/05/21 06:00
PHST- 2013/02/04 00:00 [received]
PHST- 2013/04/19 00:00 [accepted]
PHST- 2013/05/21 06:00 [entrez]
PHST- 2013/05/21 06:00 [pubmed]
PHST- 2014/04/26 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 1297-9716-44-35 [pii]
AID - 10.1186/1297-9716-44-35 [doi]
PST - epublish
SO  - Vet Res. 2013 May 17;44(1):35. doi: 10.1186/1297-9716-44-35.