PMID- 23683858
OWN - NLM
STAT- MEDLINE
DCOM- 20140331
LR  - 20181203
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 63
IP  - 1
DP  - 2013 Jul
TI  - Postprandial human triglyceride-rich lipoproteins increase chemoattractant 
      protein secretion in human macrophages.
PG  - 18-26
LID - S1043-4666(13)00190-7 [pii]
LID - 10.1016/j.cyto.2013.04.025 [doi]
AB  - This study tested the hypothesis that postprandial triglyceride-rich lipoproteins 
      (ppTGRL) have inflammatory effects in primary human monocyte-derived macrophages 
      (HMDM). ppTGRL were isolated from normolipidemic human volunteers, and the 
      production of chemokines and of inflammatory prostaglandins and leukotrienes via 
      the arachidonic acid cascade in HMDM was determined, and their effect on monocyte 
      chemotaxis were assessed. In addition, the possible role of extracellular lipases 
      in the inflammatory effects of ppTGRL was evaluated. ppTGRL were found to 
      increase the secretion of chemoattractants, including monocyte chemoattractant 
      protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1alpha and -1beta and IL-8, by 
      HMDM and to have a stimulatory effect on monocyte chemotaxis. HMDM secretion of 
      leukotrienes B4 (LTB4) and lipoxin A (LXA4), which are potent activators of 
      monocyte migration, was also stimulated by ppTGRL. Inclusion of the lipoprotein 
      lipase (LPL) inhibitor orlistat did not alter the effects of ppTGRL on chemokine 
      production, and the expression of mRNA for LPL and other secreted lipases was 
      unaffected by the lipoproteins. These findings support the hypothesis that ppTGRL 
      induce the secretion of chemokines by macrophages which promote monocyte 
      recruitment, and that extracellular lipolysis of the particles is not required 
      for these effects and provide further evidence to indicate that the postprandial 
      lipoproteins contribute to a pro-atherogenic pattern after a fat-rich meal.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Napolitano, Mariarosaria
AU  - Napolitano M
AD  - Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di 
      Sanita, Viale Regina Elena, 299, 00161 Rome, Italy.
FAU - Botham, Kathleen M
AU  - Botham KM
AD  - Department of Basic Sciences, The Royal Veterinary College, University of London, 
      Royal College Street, London, United Kingdom.
FAU - Bravo, Elena
AU  - Bravo E
AD  - Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di 
      Sanita, Viale Regina Elena, 299, 00161 Rome, Italy. Electronic address: 
      elena.bravo@iss.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130515
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Fatty Acids)
RN  - 0 (Lipoproteins)
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - 0 (Triglycerides)
RN  - 0 (lipoprotein triglyceride)
RN  - 27YG812J1I (Arachidonic Acid)
SB  - IM
MH  - Adult
MH  - Arachidonic Acid/metabolism
MH  - Chemotaxis/drug effects
MH  - Extracellular Space/drug effects/metabolism
MH  - Fatty Acids/metabolism
MH  - Female
MH  - Humans
MH  - Inflammation/pathology
MH  - Lipolysis/drug effects
MH  - Lipoproteins/*pharmacology
MH  - Macrophages/drug effects/*metabolism/pathology
MH  - Male
MH  - Monocyte Chemoattractant Proteins/*metabolism
MH  - Monocytes/pathology
MH  - Postprandial Period/*drug effects
MH  - Triglycerides/*pharmacology
EDAT- 2013/05/21 06:00
MHDA- 2014/04/01 06:00
CRDT- 2013/05/21 06:00
PHST- 2012/04/21 00:00 [received]
PHST- 2013/04/19 00:00 [revised]
PHST- 2013/04/21 00:00 [accepted]
PHST- 2013/05/21 06:00 [entrez]
PHST- 2013/05/21 06:00 [pubmed]
PHST- 2014/04/01 06:00 [medline]
AID - S1043-4666(13)00190-7 [pii]
AID - 10.1016/j.cyto.2013.04.025 [doi]
PST - ppublish
SO  - Cytokine. 2013 Jul;63(1):18-26. doi: 10.1016/j.cyto.2013.04.025. Epub 2013 May 
      15.