PMID- 23684238
OWN - NLM
STAT- MEDLINE
DCOM- 20140811
LR  - 20220330
IS  - 1878-1632 (Electronic)
IS  - 1529-9430 (Print)
IS  - 1529-9430 (Linking)
VI  - 13
IP  - 11
DP  - 2013 Nov
TI  - Rough titanium alloys regulate osteoblast production of angiogenic factors.
PG  - 1563-70
LID - S1529-9430(13)00399-9 [pii]
LID - 10.1016/j.spinee.2013.03.047 [doi]
AB  - BACKGROUND CONTEXT: Polyether-ether-ketone (PEEK) and titanium-aluminum-vanadium 
      (titanium alloy) are used frequently in lumbar spine interbody fusion. 
      Osteoblasts cultured on microstructured titanium generate an environment 
      characterized by increased angiogenic factors and factors that inhibit osteoclast 
      activity mediated by integrin alpha2beta1 signaling. It is not known if this is also 
      true of osteoblasts on titanium alloy or PEEK. PURPOSE: The purpose of this study 
      was to determine if osteoblasts generate an environment that supports 
      angiogenesis and reduces osteoclastic activity when grown on smooth titanium 
      alloy, rough titanium alloy, or PEEK. STUDY DESIGN: This in vitro study compared 
      angiogenic factor production and integrin gene expression of human 
      osteoblast-like MG63 cells cultured on PEEK or titanium-aluminum-vanadium 
      (titanium alloy). METHODS: MG63 cells were grown on PEEK, smooth titanium alloy, 
      or rough titanium alloy. Osteogenic microenvironment was characterized by 
      secretion of osteoprotegerin and transforming growth factor beta-1 (TGF-beta1), 
      which inhibit osteoclast activity and angiogenic factors including vascular 
      endothelial growth factor A (VEGF-A), fibroblast growth factor 2 (FGF-2), and 
      angiopoietin-1 (ANG-1). Expression of integrins, transmembrane extracellular 
      matrix recognition proteins, was measured by real-time polymerase chain reaction. 
      RESULTS: Culture on titanium alloy stimulated osteoprotegerin, TGF-beta1, VEGF-A, 
      FGF-2, and angiopoietin-1 production, and levels were greater on rough titanium 
      alloy than on smooth titanium alloy. All factors measured were significantly 
      lower on PEEK than on smooth or rough titanium alloy. Culture on titanium alloy 
      stimulated expression of messenger RNA for integrins that recognize Type I 
      collagen in comparison with PEEK. CONCLUSIONS: Rough titanium alloy stimulated 
      cells to create an osteogenic-angiogenic microenvironment. The 
      osteogenic-angiogenic responses to titanium alloy were greater than PEEK and 
      greater on rough titanium alloy than on smooth titanium alloy. Surface features 
      regulated expression of integrins important in collagen recognition. These 
      factors may increase bone formation, enhance integration, and improve implant 
      stability in interbody spinal fusions.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Olivares-Navarrete, Rene
AU  - Olivares-Navarrete R
AD  - Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory 
      University, Georgia Institute of Technology, 315 Ferst Drive NW, Atlanta, GA 
      30332-0363, USA.
FAU - Hyzy, Sharon L
AU  - Hyzy SL
FAU - Gittens, Rolando A 1st
AU  - Gittens RA 1st
FAU - Schneider, Jennifer M
AU  - Schneider JM
FAU - Haithcock, David A
AU  - Haithcock DA
FAU - Ullrich, Peter F
AU  - Ullrich PF
FAU - Slosar, Paul J
AU  - Slosar PJ
FAU - Schwartz, Zvi
AU  - Schwartz Z
FAU - Boyan, Barbara D
AU  - Boyan BD
LA  - eng
GR  - R01 AR052102/AR/NIAMS NIH HHS/United States
GR  - AR052102/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130514
PL  - United States
TA  - Spine J
JT  - The spine journal : official journal of the North American Spine Society
JID - 101130732
RN  - 0 (Alloys)
RN  - 0 (Angiopoietin-1)
RN  - 0 (Benzophenones)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Ketones)
RN  - 0 (Osteoprotegerin)
RN  - 0 (Polymers)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 31694-16-3 (polyetheretherketone)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Alloys/pharmacology
MH  - Angiopoietin-1/*metabolism
MH  - Benzophenones
MH  - Biocompatible Materials
MH  - Cell Line
MH  - Cells, Cultured
MH  - Fibroblast Growth Factor 2/*metabolism
MH  - Humans
MH  - Ketones/*pharmacology
MH  - Osteoblasts/*drug effects/metabolism
MH  - Osteoprotegerin/metabolism
MH  - Polyethylene Glycols/*pharmacology
MH  - Polymers
MH  - Titanium/*pharmacology
MH  - Transforming Growth Factor beta1/metabolism
MH  - Vascular Endothelial Growth Factor A/*metabolism
PMC - PMC3785549
MID - NIHMS480504
OTO - NOTNLM
OT  - Angiogenesis
OT  - Osteoblast
OT  - PEEK
OT  - Polyether-ether-ketone
OT  - Titanium-aluminum-vanadium alloy
EDAT- 2013/05/21 06:00
MHDA- 2014/08/12 06:00
PMCR- 2014/11/01
CRDT- 2013/05/21 06:00
PHST- 2012/03/03 00:00 [received]
PHST- 2012/11/28 00:00 [revised]
PHST- 2013/03/20 00:00 [accepted]
PHST- 2013/05/21 06:00 [entrez]
PHST- 2013/05/21 06:00 [pubmed]
PHST- 2014/08/12 06:00 [medline]
PHST- 2014/11/01 00:00 [pmc-release]
AID - S1529-9430(13)00399-9 [pii]
AID - 10.1016/j.spinee.2013.03.047 [doi]
PST - ppublish
SO  - Spine J. 2013 Nov;13(11):1563-70. doi: 10.1016/j.spinee.2013.03.047. Epub 2013 
      May 14.