PMID- 23687941
OWN - NLM
STAT- MEDLINE
DCOM- 20150331
LR  - 20211021
IS  - 1743-0003 (Electronic)
IS  - 1743-0003 (Linking)
VI  - 10
DP  - 2013 May 20
TI  - Early changes in muscle atrophy and muscle fiber type conversion after spinal 
      cord transection and peripheral nerve transection in rats.
PG  - 46
LID - 10.1186/1743-0003-10-46 [doi]
AB  - BACKGROUND: Spinal cord transection and peripheral nerve transection cause muscle 
      atrophy and muscle fiber type conversion. It is still unknown how spinal cord 
      transection and peripheral nerve transection each affect the differentiation of 
      muscle fiber type conversion mechanism and muscle atrophy. The aim of our study 
      was to evaluate the difference of muscle weight change, muscle fiber type 
      conversion, and Peroxisome proliferator-activated receptor-gamma coactivatior-1alpha 
      (PGC-1alpha) expression brought about by spinal cord transection and by peripheral 
      nerve transection. METHODS: Twenty-four Wistar rats underwent surgery, the 
      control rats underwent a laminectomy; the spinal cord injury group underwent a 
      spinal cord transection; the denervation group underwent a sciatic nerve 
      transection. The rats were harvested of the soleus muscle and the TA muscle at 
      0 week, 1 week and 2 weeks after surgery. Histological examination was assessed 
      using hematoxylin and eosin (H&E) staining and immunofluorescent staing. Western 
      blot was performed with 3 groups. RESULTS: Both sciatic nerve transection and 
      spinal cord transection caused muscle atrophy with the effect being more severe 
      after sciatic nerve transection. Spinal cord transection caused a reduction in 
      the expression of both sMHC protein and PGC-1alpha protein in the soleus muscle. On 
      the other hand, sciatic nerve transection produced an increase in expression of 
      sMHC protein and PGC-1alpha protein in the soleus muscle. The results of the 
      expression of PGC-1alpha were expected in other words muscle atrophy after sciatic 
      nerve transection is less than after spinal cord transection, however muscle 
      atrophy after sciatic nerve transection was more severe than after spinal cord 
      transection. CONCLUSION: In the conclusion, spinal cord transection diminished 
      the expression of sMHC protein and PGC-1alpha protein in the soleus muscle. On the 
      other hand, sciatic nerve transection enhanced the expression of sMHC protein and 
      PGC-1alpha protein in the soleus muscle.
FAU - Higashino, Kosaku
AU  - Higashino K
FAU - Matsuura, Tetsuya
AU  - Matsuura T
FAU - Suganuma, Katsuyoshi
AU  - Suganuma K
FAU - Yukata, Kiminori
AU  - Yukata K
FAU - Nishisho, Toshihiko
AU  - Nishisho T
FAU - Yasui, Natsuo
AU  - Yasui N
LA  - eng
PT  - Journal Article
DEP - 20130520
PL  - England
TA  - J Neuroeng Rehabil
JT  - Journal of neuroengineering and rehabilitation
JID - 101232233
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (Ppargc1a protein, rat)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Body Weight/physiology
MH  - Female
MH  - Muscle Fibers, Skeletal/metabolism/*pathology
MH  - Muscle, Skeletal/innervation/pathology
MH  - Muscular Atrophy/metabolism/*pathology
MH  - Organ Size/physiology
MH  - Peripheral Nerve Injuries/metabolism/*pathology
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
MH  - Rats
MH  - Rats, Wistar
MH  - Sciatic Nerve/injuries
MH  - Spinal Cord Injuries/metabolism/*pathology
MH  - Transcription Factors/metabolism
PMC - PMC3668998
EDAT- 2013/05/22 06:00
MHDA- 2015/04/01 06:00
PMCR- 2013/05/20
CRDT- 2013/05/22 06:00
PHST- 2012/06/08 00:00 [received]
PHST- 2013/03/25 00:00 [accepted]
PHST- 2013/05/22 06:00 [entrez]
PHST- 2013/05/22 06:00 [pubmed]
PHST- 2015/04/01 06:00 [medline]
PHST- 2013/05/20 00:00 [pmc-release]
AID - 1743-0003-10-46 [pii]
AID - 10.1186/1743-0003-10-46 [doi]
PST - epublish
SO  - J Neuroeng Rehabil. 2013 May 20;10:46. doi: 10.1186/1743-0003-10-46.