PMID- 23688338
OWN - NLM
STAT- MEDLINE
DCOM- 20140811
LR  - 20211021
IS  - 1476-4954 (Electronic)
IS  - 1476-7058 (Print)
IS  - 1476-4954 (Linking)
VI  - 27
IP  - 2
DP  - 2014 Jan
TI  - Soluble ST2, a modulator of the inflammatory response, in preterm and term labor.
PG  - 111-21
LID - 10.3109/14767058.2013.806894 [doi]
AB  - OBJECTIVE: Intra-amniotic infection/inflammation (IAI) is causally linked with 
      spontaneous preterm labor and delivery. The ST2L receptor and its soluble form 
      (sST2) are capable of binding to interleukin (IL)-33, a member of the IL-1 
      superfamily. Members of this cytokine family have been implicated in the onset of 
      spontaneous preterm labor in the context of infection. Soluble ST2 has 
      anti-inflammatory properties, and plasma concentrations are elevated in systemic 
      inflammation, such as sepsis, acute pyelonephritis in pregnancy and the fetal 
      inflammatory response syndrome. The aims of this study were to examine: (1) 
      whether amniotic fluid concentrations of sST2 change with IAI, preterm, and term 
      parturition; and (2) if mRNA expression of ST2 in the chorioamniotic membranes 
      changes with acute histologic chorioamnionitis in women who deliver preterm. 
      METHOD: A cross-sectional study was conducted to determine amniotic fluid 
      concentrations of sST2 in: (1) women with preterm labor (PTL) who delivered at 
      term (n=49); (2) women with PTL who delivered preterm without IAI (n=21); (3) 
      women with PTL who delivered preterm with IAI (n=31); (4) term pregnancies not in 
      labor (n=13); and (5) term pregnancies in labor (n=43). The amniotic fluid 
      concentration of sST2 was determined by ELISA. The mRNA expression of ST2 in the 
      chorioamniotic membranes of women who delivered preterm with (n=24), and without 
      acute histologic chorioamnionitis (n=19) was determined by qRT-PCR. RESULTS: (1) 
      Patients with PTL who delivered preterm with IAI had a lower median amniotic 
      fluid concentration of sST2 compared to those with PTL who delivered preterm 
      without IAI [median 410 ng/mL, inter-quartile range (IQR) 152-699 ng/mL versus 
      median 825 ng/mL, IQR 493-1216 ng/mL; p=0.0003] and those with PTL who delivered 
      at term [median 410 ng/mL, IQR 152-699 ng/mL versus median 673 ng/mL, IQR 
      468-1045 ng/mL; p=0.0003]; (2) no significant differences in the median amniotic 
      fluid concentration of sST2 were observed between patients with PTL who delivered 
      at term and those who delivered preterm without IAI (p=0.4), and between women at 
      term in labor and those at term not in labor (p=0.9); (3) the mean mRNA 
      expression of ST2 was 4-fold lower in women who delivered preterm with acute 
      histologic chorioamnionitis than in those without this lesion (p=0.008). 
      CONCLUSIONS: The median sST2 amniotic fluid concentration and mRNA expression of 
      ST2 by chorioamniotic membranes is lower in PTL associated with IAI and acute 
      histologic chorioamnionitis than in PTL without these conditions. Changes in the 
      median amniotic fluid sST2 concentration are not observed in preterm and term 
      parturition without IAI. Thus, amniotic fluid sST2 in the presence of IAI behaves 
      differently when compared to sST2 in the plasma of individuals affected by fetal 
      inflammatory response syndrome, acute pyelonephritis in pregnancy, and adult 
      sepsis. Decreased concentrations of sST2 in IAI are likely to promote a 
      pro-inflammatory response, which is important for parturition in the context of 
      infection.
FAU - Stampalija, Tamara
AU  - Stampalija T
AD  - Perinatology Research Branch, NICHD/NIH/DHHS , Bethesda, Maryland, and Detroit, 
      Michigan , USA .
FAU - Chaiworapongsa, Tinnakorn
AU  - Chaiworapongsa T
FAU - Romero, Roberto
AU  - Romero R
FAU - Tarca, Adi L
AU  - Tarca AL
FAU - Bhatti, Gaurav
AU  - Bhatti G
FAU - Chiang, Po Jen
AU  - Chiang PJ
FAU - Than, Nandor Gabor
AU  - Than NG
FAU - Ferrazzi, Enrico
AU  - Ferrazzi E
FAU - Hassan, Sonia S
AU  - Hassan SS
FAU - Yeo, Lami
AU  - Yeo L
LA  - eng
GR  - HHSN275201300006C/HD/NICHD NIH HHS/United States
GR  - ZIA HD002400-22/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131113
PL  - England
TA  - J Matern Fetal Neonatal Med
JT  - The journal of maternal-fetal & neonatal medicine : the official journal of the 
      European Association of Perinatal Medicine, the Federation of Asia and Oceania 
      Perinatal Societies, the International Society of Perinatal Obstetricians
JID - 101136916
RN  - 0 (IL1RL1 protein, human)
RN  - 0 (IL33 protein, human)
RN  - 0 (Interleukin-1 Receptor-Like 1 Protein)
RN  - 0 (Interleukin-33)
RN  - 0 (Interleukins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Amnion/chemistry
MH  - Amniotic Fluid/chemistry
MH  - Chorioamnionitis/*metabolism
MH  - Chorion/chemistry
MH  - Cross-Sectional Studies
MH  - Female
MH  - Humans
MH  - Interleukin-1 Receptor-Like 1 Protein
MH  - Interleukin-33
MH  - Interleukins/metabolism
MH  - Labor, Obstetric/*metabolism
MH  - Obstetric Labor, Premature/*metabolism
MH  - Parturition
MH  - Pregnancy
MH  - Premature Birth/metabolism
MH  - RNA, Messenger/analysis
MH  - Receptors, Cell Surface/analysis/genetics/*physiology
MH  - Retrospective Studies
PMC - PMC4161022
MID - NIHMS622168
COIS- Declaration of Interest: The authors declare no conflict of interest.
EDAT- 2013/05/22 06:00
MHDA- 2014/08/12 06:00
PMCR- 2015/01/01
CRDT- 2013/05/22 06:00
PHST- 2013/05/22 06:00 [entrez]
PHST- 2013/05/22 06:00 [pubmed]
PHST- 2014/08/12 06:00 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.3109/14767058.2013.806894 [doi]
PST - ppublish
SO  - J Matern Fetal Neonatal Med. 2014 Jan;27(2):111-21. doi: 
      10.3109/14767058.2013.806894. Epub 2013 Nov 13.