PMID- 23688614 OWN - NLM STAT- MEDLINE DCOM- 20131107 LR - 20130916 IS - 0027-5107 (Print) IS - 0027-5107 (Linking) VI - 756 IP - 1-2 DP - 2013 Aug 30 TI - Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET). PG - 101-7 LID - S1383-5718(13)00125-3 [pii] LID - 10.1016/j.mrgentox.2013.05.005 [doi] AB - We have studied the induction of chromosome aberrations in human fibroblasts exposed in G0/G1 to X-rays or heavy ions to study the influence of G1 cell cycle arrest. Confluent normal fibroblasts were exposed to X-rays or accelerated particles with different LET values and chromosome aberrations were investigated in the first G0/G1 and G2//M phase. The particles used here were 490MeV/nucleon Si, 500MeV/nucleon Fe, and 200MeV/nucleon Fe ions. Cells were subcultured 24h after exposure and premature chromosome condensation (PCC) was performed by fusion-induced method for analysis of G0/G1 cells, and chemically-induced method for analysis of G2 and metaphase cells. Chromosome damage was assessed in chromosomes 1 and 3 using whole chromosome fluorescence in situ hybridization (FISH). Cell cycle was analyzed by flow cytometry at different incubation times following subculture. After irradiation with 2Gy of high-LET particles, the yields of chromosome aberrations and fragments were significantly higher in G0/G1 phase than in G2/M phase, whereas similar yields of damage were measured in both phases after exposure to X-rays. In contrast, the yield of misrepair, assessed by the number of color junctions, was similar in the G0/G1 and G2/M phases after exposure to either X-rays or high-LET particles. The yields of chromosome aberrations, fragments, and color junctions in both the G0/G1 and the G2/M phases, increased with LET up to 200keV/mum, then decreased for 440keV/mum Fe particles. A good correlation was found between chromosome aberrations in both G0/G1 and G2/M cells and survival fractions after 2Gy of different LET radiations, although the slopes were steeper for the G0/G1 cells. Flow cytometry analysis indicated that high-LET particles induce more non cycling G0/G1 cells within 48h of subculture than X-rays, suggesting that chromosome aberrations scored at the G2/M phase may not accurately describe the true radiation effect. CI - Copyright (c) 2013 Elsevier B.V. All rights reserved. FAU - Liu, Cuihua AU - Liu C AD - Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Chiba 263-8555, Japan. FAU - Kawata, Tetsuya AU - Kawata T FAU - Furusawa, Yoshiya AU - Furusawa Y FAU - Zhou, Guangming AU - Zhou G FAU - Inoue, Kohei AU - Inoue K FAU - Fukada, Junichi AU - Fukada J FAU - Kota, Ryuichi AU - Kota R FAU - George, Kerry AU - George K FAU - Cucinotta, Francis AU - Cucinotta F FAU - Okayasu, Ryuichi AU - Okayasu R LA - eng PT - Journal Article DEP - 20130517 PL - Netherlands TA - Mutat Res JT - Mutation research JID - 0400763 SB - IM MH - Cell Cycle/*genetics/*radiation effects MH - Cells, Cultured MH - Chromosome Aberrations/*radiation effects MH - Fibroblasts/cytology/metabolism/*radiation effects MH - Flow Cytometry MH - Humans MH - Image Processing, Computer-Assisted MH - In Situ Hybridization, Fluorescence MH - Linear Energy Transfer MH - Skin/cytology/metabolism/*radiation effects MH - X-Rays OTO - NOTNLM OT - Cell cycle arrest OT - Chromosome aberration OT - Fluorescence in situ hybridization (FISH) OT - High-LET radiation OT - Premature chromosome condensation (PCC) EDAT- 2013/05/22 06:00 MHDA- 2013/11/08 06:00 CRDT- 2013/05/22 06:00 PHST- 2013/05/07 00:00 [received] PHST- 2013/05/09 00:00 [accepted] PHST- 2013/05/22 06:00 [entrez] PHST- 2013/05/22 06:00 [pubmed] PHST- 2013/11/08 06:00 [medline] AID - S1383-5718(13)00125-3 [pii] AID - 10.1016/j.mrgentox.2013.05.005 [doi] PST - ppublish SO - Mutat Res. 2013 Aug 30;756(1-2):101-7. doi: 10.1016/j.mrgentox.2013.05.005. Epub 2013 May 17.