PMID- 23688994
OWN - NLM
STAT- MEDLINE
DCOM- 20140724
LR  - 20130521
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 33
IP  - 5
DP  - 2013 May
TI  - [HAND system-based four multiplex RT-PCR for simultaneous detection of four 
      diarrhea viruses].
PG  - 724-7
AB  - OBJECTIVE: To establish a one-step four multiplex reverse transcription 
      polymerase chain reaction (RT-PCR) method based on Homo-Tag Assisted Non-Dimer 
      System (HAND) system for simultaneous detection of 4 diarrhea viruses of 
      rotavirus, astrovirus, norovirus and sapovirus. METHODS: Primers were designed 
      according to the conserved genome sequence of the 4 viruses and the homologous 
      tail sequences were added to the 5' end. The multiplex RT-PCR system was 
      constructed by optimizing the PCR parameters such as the concentration of 
      universal tag primer and genome-specific Homo-Tailed primers. The specificity, 
      stability and sensitivity of the method were evaluated systematically. RESULTS: 
      The 4 multiplex RT-PCR methods based on HAND system was established successfully. 
      Specificity analysis showed no cross reaction between the 4 diarrhea viruses. The 
      sensitivity analysis showed detection limits for rotavirus, astrovirus, norovirus 
      and sapovirus of 48, 1.92, 9.6 and 48 pg per reaction, respectively. CONCLUSION: 
      The established HAND system-based multiplex RT-PCR assay allows simple, rapid, 
      specific, sensitive, and stable for detection of the 4 common diarrhea viruses at 
      low costs and is suitable for application in general medical laboratories.
FAU - Teng, Yongyong
AU  - Teng Y
AD  - Department of Epidemiology, Southern Medical University, Guangzhou, China. 
      gztengyy@163.com
FAU - Mo, Qiuhua
AU  - Mo Q
FAU - Wang, Qi
AU  - Wang Q
FAU - Tang, Minghui
AU  - Tang M
FAU - Zhao, Dejian
AU  - Zhao D
FAU - Tan, Hua
AU  - Tan H
FAU - Tu, Chengning
AU  - Tu C
FAU - Yang, Ze
AU  - Yang Z
FAU - Chen, Qing
AU  - Chen Q
FAU - Sun, Hong
AU  - Sun H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Astroviridae/genetics/*isolation & purification
MH  - Diarrhea/*virology
MH  - Feces/virology
MH  - Humans
MH  - Multiplex Polymerase Chain Reaction/*methods
MH  - Norovirus/genetics/*isolation & purification
MH  - RNA, Viral/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Rotavirus/genetics/*isolation & purification
MH  - Sapovirus/genetics/*isolation & purification
EDAT- 2013/05/22 06:00
MHDA- 2014/07/25 06:00
CRDT- 2013/05/22 06:00
PHST- 2013/05/22 06:00 [entrez]
PHST- 2013/05/22 06:00 [pubmed]
PHST- 2014/07/25 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2013 May;33(5):724-7.