PMID- 23691069
OWN - NLM
STAT- MEDLINE
DCOM- 20131216
LR  - 20240319
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 5
DP  - 2013
TI  - Broadening of the T-cell repertoire to HIV-1 Gag p24 by vaccination of HLA-A2/DR 
      transgenic mice with overlapping peptides in the CAF05 adjuvant.
PG  - e63575
LID - 10.1371/journal.pone.0063575 [doi]
LID - e63575
AB  - Induction of broad T-cell immune responses is regarded as critical for vaccines 
      against the human immunodeficiency virus type 1 (HIV-1) which exhibit high 
      diversity and, therefore, focus has been on inducing cytotoxic CD8 T-cell 
      responses against the more conserved parts of the virus, such as the Gag protein. 
      Herein, we have used the p24 protein which contains a range of conserved T-cell 
      epitopes. We demonstrate that a vaccine of HIV-1 subtype B consensus 
      group-specific antigen (Gag) p24 protein with the CD8-inducing liposomal cationic 
      adjuvant formulation (CAF) 05, induces both CD4 and CD8 T-cell responses in CB6F1 
      mice. The adjuvanted vaccine also induced functional antigen-specific 
      cytotoxicity in vivo. Furthermore, we found that when fragmenting the Gag p24 
      protein into overlapping Gag p24 peptides, a broader T-cell epitope specificity 
      was induced in the humanized human leukocyte antigen (HLA)-A2/DR-transgenic mouse 
      model. Thus, combining overlapping Gag p24 peptides with CAF05 appears to be a 
      promising and simple strategy for inducing broader T-cell responses to multiple 
      conserved epitopes which will be relevant for both prophylactic and therapeutic 
      HIV-1 vaccines.
FAU - Korsholm, Karen S
AU  - Korsholm KS
AD  - Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, 
      Denmark.
FAU - Karlsson, Ingrid
AU  - Karlsson I
FAU - Tang, Sheila T
AU  - Tang ST
FAU - Brandt, Lea
AU  - Brandt L
FAU - Agger, Else Marie
AU  - Agger EM
FAU - Aagaard, Claus
AU  - Aagaard C
FAU - Andersen, Peter
AU  - Andersen P
FAU - Fomsgaard, Anders
AU  - Fomsgaard A
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130517
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (AIDS Vaccines)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (p24 protein, Human Immunodeficiency Virus Type 1)
SB  - IM
MH  - AIDS Vaccines/*immunology
MH  - Amino Acid Sequence
MH  - Analysis of Variance
MH  - Animals
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Enzyme-Linked Immunospot Assay
MH  - Epitopes, T-Lymphocyte/immunology
MH  - HIV Core Protein p24/genetics/*immunology
MH  - HIV-1/*immunology
MH  - HLA-A2 Antigen/genetics
MH  - Immunity, Cellular/*immunology
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
PMC - PMC3656914
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/05/22 06:00
MHDA- 2013/12/18 06:00
PMCR- 2013/05/17
CRDT- 2013/05/22 06:00
PHST- 2012/12/21 00:00 [received]
PHST- 2013/04/04 00:00 [accepted]
PHST- 2013/05/22 06:00 [entrez]
PHST- 2013/05/22 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
PHST- 2013/05/17 00:00 [pmc-release]
AID - PONE-D-12-40566 [pii]
AID - 10.1371/journal.pone.0063575 [doi]
PST - epublish
SO  - PLoS One. 2013 May 17;8(5):e63575. doi: 10.1371/journal.pone.0063575. Print 2013.