PMID- 23692092
OWN - NLM
STAT- MEDLINE
DCOM- 20130724
LR  - 20211021
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 46
IP  - 3
DP  - 2013 Jun
TI  - Elucidation of proliferative capability of mononuclear tetraploid cells, emerging 
      spontaneously from diploid cells, using image cytometry and fluorescence in situ 
      hybridization.
PG  - 356-63
LID - 10.1111/cpr.12032 [doi]
AB  - OBJECTIVES: Proliferation of tetraploid cells (TCs) emerging from diploid cells 
      is considered to be a critical event toward tumourigenesis, or cancer 
      progression. Recently, several studies have reported that binuclear TCs emerging 
      from normal cells are capable of mitosis, however, it has not been confirmed 
      directly whether mononuclear TCs emerging from normal cells could proliferate, 
      even cancer cells. The aim of this study is to detect mononuclear TCs in vitro, 
      spontaneously emerging from diploid cells and to elucidate their proliferative 
      capability directly. For this purpose, we have developed a novel method. 
      MATERIALS AND METHODS: In this study, two completely disomic cell lines were 
      used, TIG-7, a fibroblast cell line and CAL-51, a breast cancer cell line. Cells 
      were cultured on microscope slides and their DNA content was determined using an 
      image cytometer. On the same slides, chromosome numbers were scored using 
      centromere fluorescence in situ hybridization (FISH). For evaluating 
      proliferative capability of TCs, bromodeoxyuridine (BrdUrd) incorporation and 
      colony-forming ability were examined. RESULTS: Using our method, spontaneous 
      emergence of mononuclear TCs was detected in both TIG-7 and CAL-51. Colonies of 
      TIG-7 TCs were not observed, but were observed of CAL-51 TCs. CONCLUSIONS: Our 
      method enables detection of mononuclear TCs and elucidation of their 
      proliferative capability, directly; this evidence reveals that mononuclear TIG-7 
      TCs do not proliferate but that mononuclear CAL-51 TCs are able to.
CI  - (c) 2013 Blackwell Publishing Ltd.
FAU - Ito, Hideaki
AU  - Ito H
AD  - Department of Pathology, Yamaguchi University Graduate School of Medicine, Ube 
      7558505, Japan.
FAU - Oga, Atsunori
AU  - Oga A
FAU - Furuya, Tomoko
AU  - Furuya T
FAU - Ikemoto, Kenzo
AU  - Ikemoto K
FAU - Amakawa, Genta
AU  - Amakawa G
FAU - Chochi, Yasuyo
AU  - Chochi Y
FAU - Kawauchi, Shigeto
AU  - Kawauchi S
FAU - Sasaki, Kohsuke
AU  - Sasaki K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
SB  - IM
MH  - Cell Line
MH  - *Cell Proliferation
MH  - *Diploidy
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Image Cytometry
MH  - In Situ Hybridization, Fluorescence
MH  - Neoplasms/*metabolism
MH  - *Tetraploidy
MH  - Tumor Cells, Cultured
PMC - PMC6496363
EDAT- 2013/05/23 06:00
MHDA- 2013/07/25 06:00
PMCR- 2013/05/22
CRDT- 2013/05/23 06:00
PHST- 2013/01/11 00:00 [received]
PHST- 2013/01/18 00:00 [accepted]
PHST- 2013/05/23 06:00 [entrez]
PHST- 2013/05/23 06:00 [pubmed]
PHST- 2013/07/25 06:00 [medline]
PHST- 2013/05/22 00:00 [pmc-release]
AID - CPR12032 [pii]
AID - 10.1111/cpr.12032 [doi]
PST - ppublish
SO  - Cell Prolif. 2013 Jun;46(3):356-63. doi: 10.1111/cpr.12032.