PMID- 23694790
OWN - NLM
STAT- MEDLINE
DCOM- 20131025
LR  - 20130522
IS  - 1550-8080 (Electronic)
IS  - 0091-7370 (Linking)
VI  - 43
IP  - 2
DP  - 2013 Spring
TI  - Intrathecal transplantation of neural stem cells appears to alleviate neuropathic 
      pain in rats through release of GDNF.
PG  - 154-62
AB  - The beneficial effect of stem cells on several animal models of neuropathic pain 
      has been recognized recently. However, there have been no studies on the effects 
      of neural stem cell transplantation on neuropathic pain. In the present study, 
      using the rat model of chronic constriction injury of the sciatic nerve, we 
      observed the effect of intrathecal transplantation of extrinsic neural stem cells 
      on the behavior and expression of neurotrophins such as brain-derived 
      neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) 
      in the spinal dorsal horn and dorsal root ganglia (DRG), and analyzed BDNF and 
      GDNF protein levels secreted by neural stem cells in vitro before and after 
      differentiation. We found that intrathecal administration of neural stem cells 3 
      days after injury could significantly attenuate mechanical and thermal 
      hyperalgesia. Furthermore, real-time PCR and enzyme-linked immunosorbent assay 
      (ELISA) results together showed that the expression of GDNF, rather than BDNF, 
      was significantly elevated in the neural stem cell transplantation group. The in 
      vitro ELISA results showed that after differentiation, GDNF secrection was 
      dramatically increased, whereas BDNF expression was sharply down-regulated. We 
      therefore concluded that the secretion of GDNF, but not BDNF, by the transplanted 
      neural stem cells might contribute to the relief of neuropathic pain.
FAU - Xu, Qian
AU  - Xu Q
AD  - Department of Anesthesiology, Jinling Hospital, Medical School of Nanjing 
      University, Nanjing, Jiangsu, China.
FAU - Zhang, Minhao
AU  - Zhang M
FAU - Liu, Jian
AU  - Liu J
FAU - Li, Weiyan
AU  - Li W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
RN  - 0 (DNA Primers)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - DNA Primers/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glial Cell Line-Derived Neurotrophic Factor/*metabolism
MH  - Hyperalgesia/physiopathology
MH  - Injections, Spinal
MH  - Male
MH  - Neural Stem Cells/metabolism/*transplantation
MH  - Neuralgia/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - brain-derived neurotrophic factor
OT  - chronic constrction injury
OT  - glial cell line-derived neurotrophic factor
OT  - neural stem cell
OT  - spinal dorsal horn
EDAT- 2013/05/23 06:00
MHDA- 2013/10/26 06:00
CRDT- 2013/05/23 06:00
PHST- 2013/05/23 06:00 [entrez]
PHST- 2013/05/23 06:00 [pubmed]
PHST- 2013/10/26 06:00 [medline]
AID - 43/2/154 [pii]
PST - ppublish
SO  - Ann Clin Lab Sci. 2013 Spring;43(2):154-62.