PMID- 23696408
OWN - NLM
STAT- MEDLINE
DCOM- 20160423
LR  - 20220321
IS  - 1671-0274 (Print)
IS  - 1671-0274 (Linking)
VI  - 16
IP  - 5
DP  - 2013 May
TI  - [Effects of esophageal cancer on the nerve fiber growth and guidance].
PG  - 474-8
AB  - OBJECTIVE: To establish the co-culture model of cancer cells and nerve, and to 
      study the influence of esophageal cancer on nerve fibers. METHODS: Mouse dorsal 
      root ganglion (DRG) was cultured in sterile conditions by primary isolation. 
      Co-culture model was established using matrigel matrix-embedded DRG and EC109 
      (esophageal cancer cell line) transfected with green fluorescent protein. 
      Morphological changes of DRG, number and area of neurites were quantified with 
      microscopy and image analysis. Furthermore, the mRNA expression of nerve growth 
      factor(NGF) and brain derived neurotrophic factor(BDNF) was detected by real-time 
      PCR. RESULTS: In mixture cultivation model of EC109 and DRG cells, directional 
      outgrowth of neurite projecting to EC109 was observed, and the length of neurite 
      was markedly longer in proximal field compared to distal field. The number and 
      area of neurite were 87 and 346 mum(2) in proximal field respectively, and 23 and 
      141 mum(2) in distal field on the 7th day. The expressions of NGF and BDNF were 
      up-regulated in esophageal cancer cells. CONCLUSIONS: The esophageal cancer may 
      play an important role in nerve fiber growth and guidance, which may be 
      associated with the up-regulation of NGF and BDNF expressions.
FAU - Xu, Guang-Hui
AU  - Xu GH
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      The Fourth Military Medical University, Xi'an 710032, China. zhanghw@fmmu.edu.cn.
FAU - Feng, Fan
AU  - Feng F
FAU - Zhao, Guo-Hong
AU  - Zhao GH
FAU - Kang, Jian-Qin
AU  - Kang JQ
FAU - Yang, Xue-Wen
AU  - Yang XW
FAU - Chen, Kang
AU  - Chen K
FAU - Guo, Yong
AU  - Guo Y
FAU - Zhang, Hong-Wei
AU  - Zhang HW
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Wei Chang Wai Ke Za Zhi
JT  - Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery
JID - 101177990
SB  - IM
MH  - Animals
MH  - Coculture Techniques
MH  - *Esophageal Neoplasms/metabolism
MH  - *Ganglia, Spinal
MH  - Humans
MH  - Neurites
MH  - Rats, Sprague-Dawley
MH  - Up-Regulation
EDAT- 2013/05/23 06:00
MHDA- 2016/04/24 06:00
CRDT- 2013/05/23 06:00
PHST- 2013/05/23 06:00 [entrez]
PHST- 2013/05/23 06:00 [pubmed]
PHST- 2016/04/24 06:00 [medline]
AID - 100001882012 [pii]
PST - ppublish
SO  - Zhonghua Wei Chang Wai Ke Za Zhi. 2013 May;16(5):474-8.