PMID- 23696606
OWN - NLM
STAT- MEDLINE
DCOM- 20130812
LR  - 20211021
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 54
IP  - 6
DP  - 2013 Jun 10
TI  - Transmission electron microscopy analysis of epithelial basement membrane repair 
      in rabbit corneas with haze.
PG  - 4026-33
LID - 10.1167/iovs.13-12106 [doi]
AB  - PURPOSE: To assess the ultrastructure of the epithelial basement membrane using 
      transmission electron microscopy (TEM) in rabbit corneas with and without 
      subepithelial stroma opacity (haze). METHODS: Two groups of eight rabbits each 
      were included in this study. Photorefractive keratectomy (PRK) was performed 
      using an excimer laser. The first group had -4.5-diopter (-4.5D) PRK and the 
      second group had -9.0D PRK. Contralateral eyes were unwounded controls. Rabbits 
      were sacrificed at 4 weeks after surgery. Immunohistochemical analysis was 
      performed to detect the myofibroblast marker alpha-smooth muscle actin (SMA). TEM was 
      performed to analyze the ultrastructure of the epithelial basement membrane and 
      stroma. RESULTS: At 4 weeks after PRK, alpha-SMA+ myofibroblasts were present at high 
      density in the subepithelial stroma of rabbit eyes that had -9.0D PRK, along with 
      prominent disorganized extracellular matrix, whereas few myofibroblasts and 
      little disorganized extracellular matrix were noted in eyes that had -4.5D PRK. 
      The epithelial basement membrane was irregular and discontinuous and lacking 
      typical morphology in all corneas at 1 month after -9D PRK compared to corneas at 
      1 month in the -4.5D PRK group. CONCLUSIONS: The epithelial basement membrane 
      acts as a critical modulator of corneal wound healing. Structural and functional 
      defects in the epithelial basement membrane correlate to both stromal 
      myofibroblast development from precursor cells and continued myofibroblast 
      viability, likely through the modulation of epithelial-stromal interactions 
      mediated by cytokines. Prolonged stromal haze in the cornea is associated with 
      abnormal regeneration of the epithelial basement membrane.
FAU - Torricelli, Andre A M
AU  - Torricelli AA
AD  - Cole Eye Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
FAU - Singh, Vivek
AU  - Singh V
FAU - Agrawal, Vandana
AU  - Agrawal V
FAU - Santhiago, Marcony R
AU  - Santhiago MR
FAU - Wilson, Steven E
AU  - Wilson SE
LA  - eng
GR  - R01 EY010056/EY/NEI NIH HHS/United States
GR  - R24 EY015638/EY/NEI NIH HHS/United States
GR  - EY10056/EY/NEI NIH HHS/United States
GR  - EY015638/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130610
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Actins)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Basement Membrane/*diagnostic imaging/metabolism/pathology
MH  - Cell Count
MH  - Corneal Stroma/*diagnostic imaging/metabolism/pathology
MH  - Disease Models, Animal
MH  - Epithelium, Corneal/*diagnostic imaging/metabolism/pathology
MH  - Female
MH  - *Microscopy, Electron, Transmission
MH  - Myofibroblasts/diagnostic imaging/metabolism/pathology
MH  - Photorefractive Keratectomy/*adverse effects
MH  - Rabbits
MH  - Regeneration/physiology
MH  - Ultrasonography
MH  - Wound Healing/physiology
PMC - PMC3680005
OTO - NOTNLM
OT  - basement membrane
OT  - corneal epithelium
OT  - corneal wound healing
OT  - haze
OT  - myofibroblasts
EDAT- 2013/05/23 06:00
MHDA- 2013/08/13 06:00
PMCR- 2013/12/01
CRDT- 2013/05/23 06:00
PHST- 2013/05/23 06:00 [entrez]
PHST- 2013/05/23 06:00 [pubmed]
PHST- 2013/08/13 06:00 [medline]
PHST- 2013/12/01 00:00 [pmc-release]
AID - iovs.13-12106 [pii]
AID - 10.1167/iovs.13-12106 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2013 Jun 10;54(6):4026-33. doi: 10.1167/iovs.13-12106.