PMID- 23700277
OWN - NLM
STAT- MEDLINE
DCOM- 20140203
LR  - 20231213
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Linking)
VI  - 21
IP  - 4
DP  - 2013 Jul
TI  - Tobacco karyotyping by accurate centromere identification and novel repetitive 
      DNA localization.
PG  - 375-81
LID - 10.1007/s10577-013-9363-y [doi]
AB  - Tobacco (Nicotiana tabacum) is an amphidiploid species (2n = 4x = 48, genome 
      constitution SSTT) derived from a natural hybrid between Nicotiana sylvestris 
      (2n = 2x = 24, SS) and Nicotiana tomentosiformis (2n = 2x = 24, TT). Genomic in 
      situ hybridization (GISH), using the genomic DNA from these ancestral species as 
      probes, revealed the chromosomal origins (S or T) and the occurrence of 
      intergenomic translocations in N. tabacum. Fluorescence in situ hybridization 
      (FISH) was also used to distinguish between chromosomes. However, the use of 
      repetitive DNA sequences as probes for FISH analysis is limited by an inability 
      to identify all chromosomes. In addition to this limitation, the occurrence of 
      chromosomal tertiary constrictions can easily lead to the misclassification of 
      chromosomes. To overcome these issues, immunostaining with anti-N. tabacum 
      centromere-specific histone H3 antibody was carried out to determine the 
      centromere position of each chromosome, followed by FISH analysis with ten 
      distinct repetitive DNA probes. This approach allowed us to identify 22 of the 24 
      chromosome pairs in N. tabacum and revealed novel intergenomic chromosome 
      rearrangements and B-chromosome-like minichromosomes. Hence, the combination of 
      immunostaining with FISH and GISH is critical to accurately karyotype tobacco.
FAU - Shibata, Fukashi
AU  - Shibata F
AD  - Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, 
      Japan.
FAU - Nagaki, Kiyotaka
AU  - Nagaki K
FAU - Yokota, Etsuko
AU  - Yokota E
FAU - Murata, Minoru
AU  - Murata M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130523
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Centromere/chemistry/*genetics
MH  - Chromosomes, Plant/genetics
MH  - DNA Probes/genetics
MH  - DNA, Plant/analysis/*genetics
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/*methods
MH  - Repetitive Sequences, Nucleic Acid/*drug effects
MH  - Nicotiana/*genetics
EDAT- 2013/05/24 06:00
MHDA- 2014/02/04 06:00
CRDT- 2013/05/24 06:00
PHST- 2013/02/06 00:00 [received]
PHST- 2013/04/30 00:00 [accepted]
PHST- 2013/04/18 00:00 [revised]
PHST- 2013/05/24 06:00 [entrez]
PHST- 2013/05/24 06:00 [pubmed]
PHST- 2014/02/04 06:00 [medline]
AID - 10.1007/s10577-013-9363-y [doi]
PST - ppublish
SO  - Chromosome Res. 2013 Jul;21(4):375-81. doi: 10.1007/s10577-013-9363-y. Epub 2013 
      May 23.