PMID- 23701883
OWN - NLM
STAT- MEDLINE
DCOM- 20130910
LR  - 20190605
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Linking)
VI  - 380
IP  - 2
DP  - 2013 Aug 15
TI  - Temporal regulation of single-minded target genes in the ventral midline of the 
      Drosophila central nervous system.
PG  - 335-43
LID - S0012-1606(13)00260-1 [pii]
LID - 10.1016/j.ydbio.2013.05.015 [doi]
AB  - Differentiation of a specific organ or tissue requires sequential activation of 
      regulatory genes. However, little is known about how serial gene expression is 
      temporally regulated. Here, we present evidence that differential expression of 
      single-minded (sim) target genes can be attributed, in part, to the number of Sim 
      and Tango (Tgo) heterodimer binding sites within their enhancer regions. The Sim, 
      termed a master regulator, directs ventral midline differentiation of Drosophila 
      central nervous system (CNS). According to data on the onset timing of ventral 
      midline gene expression, sim target genes are classified into at least 2 groups 
      (early and late). The sim and rhomboid (rho) genes are activated during early 
      midline differentiation whereas orthodenticle (otd), CG10249, and slit (sli) 
      genes undergo activation during later stages of midline differentiation. Germline 
      transformation and in situ hybridization with transgenic embryos demonstrate that 
      enhancers activating sim and rho expression contain 4 Sim-Tgo binding sites 
      whereas only 1 Sim-Tgo binding site is found in an enhancer of sli. A mutagenized 
      version of the rho enhancer lacking either 1, 2, or 3 Sim-Tgo binding sites 
      mediated progressively more delayed expression of a lacZ reporter gene in the 
      ventral midline. In contrast, a modified sli enhancer displayed progressively 
      earlier onset of lacZ expression when 1, 2, or 3 more Sim-Tgo binding sites were 
      added. Taken together, these results suggest that the number of Sim-Tgo-binding 
      sites is decisive in determining the timing of gene expression in the developing 
      ventral midline. We also discuss a combinatorial model accounting for the 
      sequential expression of sim target genes.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Hong, Joung-Woo
AU  - Hong JW
AD  - Graduate School of East-West Medical Science, Kyung Hee University, Yongin 
      446-701, South Korea. jwhong46@khu.ac.kr
FAU - Park, Kye Won
AU  - Park KW
FAU - Levine, Michael S
AU  - Levine MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130520
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Rho protein, Drosophila)
RN  - 0 (sim protein, Drosophila)
RN  - 0 (tgo protein, Drosophila)
RN  - 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator)
SB  - IM
MH  - Animals
MH  - Aryl Hydrocarbon Receptor Nuclear Translocator/genetics
MH  - Basic Helix-Loop-Helix Transcription Factors/*genetics
MH  - Binding Sites
MH  - Cell Differentiation
MH  - Central Nervous System/*embryology
MH  - Drosophila Proteins/*genetics
MH  - Drosophila melanogaster/*embryology
MH  - *Gene Expression Regulation, Developmental
MH  - Membrane Proteins/genetics
MH  - Nuclear Proteins/*genetics
MH  - SELEX Aptamer Technique
OTO - NOTNLM
OT  - Drosophila
OT  - Embryo
OT  - Single-minded
OT  - Temporal regulation
OT  - Ventral midline
EDAT- 2013/05/25 06:00
MHDA- 2013/09/11 06:00
CRDT- 2013/05/25 06:00
PHST- 2013/03/14 00:00 [received]
PHST- 2013/05/11 00:00 [revised]
PHST- 2013/05/13 00:00 [accepted]
PHST- 2013/05/25 06:00 [entrez]
PHST- 2013/05/25 06:00 [pubmed]
PHST- 2013/09/11 06:00 [medline]
AID - S0012-1606(13)00260-1 [pii]
AID - 10.1016/j.ydbio.2013.05.015 [doi]
PST - ppublish
SO  - Dev Biol. 2013 Aug 15;380(2):335-43. doi: 10.1016/j.ydbio.2013.05.015. Epub 2013 
      May 20.