PMID- 23702255
OWN - NLM
STAT- MEDLINE
DCOM- 20140207
LR  - 20160415
IS  - 1532-2785 (Electronic)
IS  - 0143-4179 (Linking)
VI  - 47
IP  - 4
DP  - 2013 Aug
TI  - Cholecystokinin-8 induces brain-derived neurotrophic factor expression in 
      noradrenergic neuronal cells.
PG  - 245-50
LID - S0143-4179(13)00025-5 [pii]
LID - 10.1016/j.npep.2013.04.002 [doi]
AB  - The sulfated cholecystokinin octapeptide (CCK-8S) is one of the most abundant CCK 
      fragment in the brain, but the effects of CCK-8S on locus coeruleus (LC) 
      noradrenergic (NA) neuronal cells activity have not been studied. In this study, 
      we investigated the effects of CCK-8S on the expression of brain-derived 
      neurotrophic factor (BDNF) in LC NA neuronal cell line, LC3541. Results showed 
      that CCK-8S (10 nM) elevates BDNF levels time-dependently and by 1.82-fold after 
      4h of incubation. In addition, pretreatment with CCK-8S reversed H(2)O(2) (100 
      muM)-mediated down-regulation of BDNF expression, and effectively suppressed 
      H(2)O(2)-induced caspase-3 activation. Furthermore, CCK-8S markedly induced 
      expression of neuronal survival markers, such as extracellular signal-regulated 
      kinase 1/2 (ERK 1/2), Akt/protein kinase B (PKB), Bcl-2, and peroxisome 
      proliferators-activated receptor gamma coactivator-1alpha (PGC-1alpha). Pharmacological 
      inhibitors of ERK 1/2, Akt/PKB, and protein kinase A (PKA) reversed 
      CCK-8S-mediated BDNF induction in LC3541 cells. These results suggest the first 
      evidence that CCK-8S can protect noradrenergic neurons and enhance the expression 
      of BDNF via ERK 1/2-Akt/PKB-PKA-dependent pathways.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Hwang, Cheol Kyu
AU  - Hwang CK
AD  - Department of Pharmacology, University of Minnesota Medical School, Minneapolis, 
      MN 55455, USA.
FAU - Kim, Do Kyung
AU  - Kim DK
FAU - Chun, Hong Sung
AU  - Chun HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130520
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Peptide Fragments)
RN  - 0 (cholecystokinin 8)
RN  - 9011-97-6 (Cholecystokinin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Adrenergic Neurons/*drug effects/metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Caspase 3/metabolism
MH  - Cell Line
MH  - Cholecystokinin/*pharmacology
MH  - Down-Regulation/drug effects
MH  - Hydrogen Peroxide/pharmacology
MH  - Membrane Potentials/drug effects
MH  - Mice
MH  - Peptide Fragments/*pharmacology
MH  - Signal Transduction/drug effects
EDAT- 2013/05/25 06:00
MHDA- 2014/02/08 06:00
CRDT- 2013/05/25 06:00
PHST- 2012/12/13 00:00 [received]
PHST- 2013/04/05 00:00 [revised]
PHST- 2013/04/18 00:00 [accepted]
PHST- 2013/05/25 06:00 [entrez]
PHST- 2013/05/25 06:00 [pubmed]
PHST- 2014/02/08 06:00 [medline]
AID - S0143-4179(13)00025-5 [pii]
AID - 10.1016/j.npep.2013.04.002 [doi]
PST - ppublish
SO  - Neuropeptides. 2013 Aug;47(4):245-50. doi: 10.1016/j.npep.2013.04.002. Epub 2013 
      May 20.