PMID- 23704927
OWN - NLM
STAT- MEDLINE
DCOM- 20131230
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 5
DP  - 2013
TI  - Alterations of serum levels of BDNF-related miRNAs in patients with depression.
PG  - e63648
LID - 10.1371/journal.pone.0063648 [doi]
LID - e63648
AB  - Depression is a serious and potentially life-threatening mental disorder with 
      unknown etiology. Emerging evidence shows that brain-derived neurotrophic factor 
      (BDNF) and microRNAs (miRNAs) play critical roles in the etiology of depression. 
      Here this study was aimed to identify and characterize the roles of BDNF and its 
      putative regulatory miRNAs in depression. First, we identified that miR-182 may 
      be a putative miRNA that regulates BDNF levels by bioinformatic studies, and 
      characterized the effects of miR-182 on the BDNF levels using cell-based studies, 
      side by side with miR-132 (a known miRNA that regulates BDNF expression). We 
      showed that treatment of miR-132 and miR-182 respectively decreased the BDNF 
      protein levels in a human neuronal cell model, supporting the regulatory roles of 
      miR-132 and miR-182 on the BDNF expression. Furthermore, we explored the roles of 
      miR-132 and miR-182 on the BDNF levels in depression using human subjects by 
      assessing their serum levels. Compared with the healthy controls, patients with 
      depression showed lower serum BDNF levels (via the enzyme-linked immunosorbent 
      assays) and higher serum miR-132 and miR-182 levels (via the real-time PCR). 
      Finally, the Pearson's (or Spearman's) correlation coefficient was calculated to 
      study whether there was a relationship among the Self-Rating Depression Scale 
      score, the serum BDNF levels, and serum BDNF-related miRNA levels. Our results 
      revealed that there was a significant negative correlation between the SDS scores 
      and the serum BDNF levels, and a positive correlation between the SDS scores and 
      miR-132 levels. In addition, we found a reverse relationship between the serum 
      BDNF levels and the miR-132/miR-182 levels in depression. Collectively, we 
      provided evidence supporting that miR-182 is a putative BDNF-regulatory miRNA, 
      and suggested that the serum BDNF and its related miRNAs may be utilized as 
      important biomarkers in the diagnosis or as therapeutic targets of depression.
FAU - Li, You-Jie
AU  - Li YJ
AD  - Department of Biochemistry and Molecular Biology, Binzhou Medical University, 
      YanTai, ShanDong, PR China.
FAU - Xu, Mei
AU  - Xu M
FAU - Gao, Zong-Hua
AU  - Gao ZH
FAU - Wang, Ya-Qi
AU  - Wang YQ
FAU - Yue, Zhen
AU  - Yue Z
FAU - Zhang, Yan-Xia
AU  - Zhang YX
FAU - Li, Xin-Xin
AU  - Li XX
FAU - Zhang, Can
AU  - Zhang C
FAU - Xie, Shu-Yang
AU  - Xie SY
FAU - Wang, Ping-Yu
AU  - Wang PY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130521
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MicroRNAs)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Adult
MH  - Brain-Derived Neurotrophic Factor/*blood/*genetics
MH  - Case-Control Studies
MH  - Demography
MH  - Depression/*blood/*genetics
MH  - Female
MH  - Humans
MH  - Male
MH  - MicroRNAs/genetics/*metabolism
MH  - Psychiatric Status Rating Scales
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC3660391
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/05/25 06:00
MHDA- 2014/01/01 06:00
PMCR- 2013/05/21
CRDT- 2013/05/25 06:00
PHST- 2012/11/20 00:00 [received]
PHST- 2013/04/04 00:00 [accepted]
PHST- 2013/05/25 06:00 [entrez]
PHST- 2013/05/25 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
PHST- 2013/05/21 00:00 [pmc-release]
AID - PONE-D-12-36622 [pii]
AID - 10.1371/journal.pone.0063648 [doi]
PST - epublish
SO  - PLoS One. 2013 May 21;8(5):e63648. doi: 10.1371/journal.pone.0063648. Print 2013.