PMID- 23704937
OWN - NLM
STAT- MEDLINE
DCOM- 20131230
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 5
DP  - 2013
TI  - Cryptoporus volvatus extract inhibits porcine reproductive and respiratory 
      syndrome virus (PRRSV) in vitro and in vivo.
PG  - e63767
LID - 10.1371/journal.pone.0063767 [doi]
LID - e63767
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) is an important 
      arterivirus that can cause significant losses in swine industry. At present, 
      there are no adequate control strategies against PRRSV. Thus, there is an urgent 
      need for new treatment regimens that have efficacious antiviral activity to 
      compensate for vaccines. Cryptoporus volvatus commonly serves as an 
      anti-infective agent in Tradational Chinese Medicines. In this report, we 
      exploited whether the aqueous extract from the fruiting body of Cryptoporus 
      volvatus had the potential to inhibit PRRSV infection. Our results showed that 
      the extract significantly inhibited PRRSV infection by repressing virus entry, 
      viral RNA expression, and possibly viral protein synthesis, cell-to-cell spread, 
      and releasing of virus particles. However, it did not block PRRSV binding to 
      cells. Further studies confirmed that the extract directly inhibited PRRSV 
      RNA-dependent RNA polymerase (RdRp) activity, thus interfering with PRRSV RNA and 
      protein synthesis. More importantly, the extract efficiently inhibited highly 
      pathologic PRRSV (HP-PRRSV) infection in vivo, reduced virus load in serum, and 
      increased the survival rate of pigs inoculated with HP-PRRSV strain. 
      Collectively, our findings imply that the aqueous extract from the fruiting body 
      of Cryptoporus volvatus has the potential to be used for anti-PRRSV therapies.
FAU - Gao, Li
AU  - Gao L
AD  - State Key Laboratory of Agrobiotechnology, China Agricultural University, 
      Beijing, China.
FAU - Zhang, Weiwei
AU  - Zhang W
FAU - Sun, Yipeng
AU  - Sun Y
FAU - Yang, Qian
AU  - Yang Q
FAU - Ren, Jie
AU  - Ren J
FAU - Liu, Jinhua
AU  - Liu J
FAU - Wang, Hexiang
AU  - Wang H
FAU - Feng, Wen-Hai
AU  - Feng WH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130521
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antiviral Agents)
RN  - 0 (Complex Mixtures)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Agaricales/*chemistry
MH  - Animals
MH  - Antiviral Agents/*pharmacology
MH  - Cell Line
MH  - Complex Mixtures/*pharmacology
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects
MH  - Protein Biosynthesis/drug effects
MH  - RNA, Viral/biosynthesis
MH  - Sus scrofa/virology
MH  - Swine
MH  - Viral Proteins/biosynthesis
MH  - Virion/drug effects/metabolism
MH  - Virus Attachment/drug effects
MH  - Virus Internalization/drug effects
MH  - Virus Replication/drug effects
PMC - PMC3660591
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/05/25 06:00
MHDA- 2014/01/01 06:00
PMCR- 2013/05/21
CRDT- 2013/05/25 06:00
PHST- 2012/12/28 00:00 [received]
PHST- 2013/04/02 00:00 [accepted]
PHST- 2013/05/25 06:00 [entrez]
PHST- 2013/05/25 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
PHST- 2013/05/21 00:00 [pmc-release]
AID - PONE-D-13-00410 [pii]
AID - 10.1371/journal.pone.0063767 [doi]
PST - epublish
SO  - PLoS One. 2013 May 21;8(5):e63767. doi: 10.1371/journal.pone.0063767. Print 2013.