PMID- 23706210
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20161125
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 33
IP  - 6
DP  - 2013 Aug 1
TI  - In vitro human periodontal ligament-like tissue formation with porous 
      poly-L-lactide matrix.
PG  - 3273-80
LID - S0928-4931(13)00222-1 [pii]
LID - 10.1016/j.msec.2013.04.008 [doi]
AB  - This study aimed to establish an in vitro human periodontal ligament-like tissue 
      (HPdLLT) by three-dimensional culturing of human periodontal ligament fibroblasts 
      (HPdLFs) in a porous poly-L-lactide (PLLA) matrix modified hydrophilically with 
      ammonia solution. After ammonia modification, the surface roughness and 
      culture-medium-soaking-up ability of the PLLA matrix increased, whereas the 
      contact angle of water drops decreased. The thickness, porosity, and pore size of 
      the PLLA matrix were 400+/-50 mum, 83.3%, and 75-150 mum, respectively. HPdLFs 
      (1x10(5) cells) were seeded on the modified PLLA matrix and centrifuged to 
      facilitate seeding into its interior and cultured for 14 days. Scanning electron 
      microscope (SEM) observation, proliferation assay, picrosirius-red staining, and 
      real-time polymerase chain reaction (RT-PCR) for type-1 collagen (COL1), 
      periodontal ligament associated protein-1 (PLAP-1), fibroblast growth factor-2 
      (FGF-2), and alkaline phosphatase (ALP) mRNA were conducted on days 1, 3, 7, and 
      14. HPdLFs were observed entirely from the surface to the rear side of the 
      matrix. Cell proliferation analysis, SEM observation, and picrosirius-red 
      staining showed both progressive growth of 3D-cultured HPdLFs and extracellular 
      matrix maturation by the secretion of COL1 and type 3 collagen (COL3) from days 1 
      to 14. Expressions of COL1, PLAP-1, and FGF-2 mRNA suggested the formation of 
      cellular components and supplementation of extracellular components. Expressions 
      of ALP, COL1, and PLAP-1 mRNA suggested the osteogenic potential of the HPdLLT. 
      The results indicated in vitro HPdLLT formation, and it could be used in future 
      periodontal ligament tissue engineering to achieve optimal periodontal 
      regeneration.
CI  - Copyright (c) 2013. Published by Elsevier B.V.
FAU - Liao, Wen
AU  - Liao W
AD  - Graduate School of Dentistry, Department of Orthodontics, Osaka Dental 
      University, Hirakata-shi, Osaka-fu, Japan.
FAU - Okada, Masahiro
AU  - Okada M
FAU - Sakamoto, Fumito
AU  - Sakamoto F
FAU - Okita, Naoya
AU  - Okita N
FAU - Inami, Kaoru
AU  - Inami K
FAU - Nishiura, Aki
AU  - Nishiura A
FAU - Hashimoto, Yoshiya
AU  - Hashimoto Y
FAU - Matsumoto, Naoyuki
AU  - Matsumoto N
LA  - eng
PT  - Journal Article
DEP - 20130411
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (ASPN protein, human)
RN  - 0 (Collagen Type I)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Polyesters)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 7664-41-7 (Ammonia)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/genetics/metabolism
MH  - Ammonia/chemistry
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - Collagen Type I/genetics/metabolism
MH  - Extracellular Matrix Proteins/genetics/metabolism
MH  - Fibroblast Growth Factor 2/genetics/metabolism
MH  - Fibroblasts/cytology/metabolism
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - Periodontal Ligament/cytology
MH  - Polyesters/*chemistry
MH  - Porosity
MH  - *Tissue Engineering
EDAT- 2013/05/28 06:00
MHDA- 2014/01/01 06:00
CRDT- 2013/05/28 06:00
PHST- 2013/01/10 00:00 [received]
PHST- 2013/03/26 00:00 [revised]
PHST- 2013/04/04 00:00 [accepted]
PHST- 2013/05/28 06:00 [entrez]
PHST- 2013/05/28 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
AID - S0928-4931(13)00222-1 [pii]
AID - 10.1016/j.msec.2013.04.008 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2013 Aug 1;33(6):3273-80. doi: 
      10.1016/j.msec.2013.04.008. Epub 2013 Apr 11.