PMID- 23707347
OWN - NLM
STAT- MEDLINE
DCOM- 20140228
LR  - 20171116
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 176
IP  - 1-2
DP  - 2013 Sep
TI  - Porcine reproductive and respiratory syndrome virus infection is associated with 
      an increased number of Sn-positive and CD8-positive cells in the maternal-fetal 
      interface.
PG  - 285-91
LID - S0168-1702(13)00170-6 [pii]
LID - 10.1016/j.virusres.2013.05.005 [doi]
AB  - It is already known that porcine reproductive and respiratory syndrome virus 
      (PRRSV) infection in lungs changes a local cell pattern and cytokine profile. 
      However, there is no information about cellular and immunological events upon 
      PRRSV infection in the maternal-fetal interface yet. The altered number and/or 
      function of macrophages and NK cells in the maternal-fetal interface during 
      infection may have a functional importance for virus replication. In addition, 
      local cellular and immunological disbalance may also disrupt fragile homeostasis 
      and contribute to the PRRSV-related reproductive disorders. Sialoadhesin 
      (Sn)-positive macrophages are target cells for PRRSV and Sn overexpression has 
      been observed upon chronic inflammatory and infectious diseases. It is also known 
      that mouse Sn-positive macrophages in lymph nodes are able to closely interact 
      with and activate NK cells in response to viral particles. Therefore, the main 
      purpose of the present study was to examine if PRRSV infection is associated with 
      altered Sn expression on endometrial and placental macrophages. In addition, 
      CD8-positive cells (porcine endometrial NK cells were previously described as 
      CD8(+)CD3(-) cells) were localized and quantified in the PRRSV-positive and 
      control tissues. Tissue samples were obtained from three PRRSV-inoculated and 
      three non-inoculated control sows at 100 days of gestation. Real-time RT-PCR 
      showed a clear upregulation of Sn mRNA expression in the PRRSV-positive 
      endometrium/placenta (p<0.05). Sn-, CD163- and CD14-specific immunofluorescence 
      stainings revealed that PRRSV-inoculated sows had a significantly higher number 
      of Sn-positive macrophages in the endometrium and placenta due to de novo Sn 
      expression on local CD163-positive macrophages. Along with the increased number 
      of Sn-positive macrophages an increased number of CD8-positive cells, which were 
      mostly CD3-negative, was observed in the PRRSV-positive endometrium. The effects 
      of the observed cellular changes on virus replication and potential contribution 
      to placental damage and reproductive disorders are discussed.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Karniychuk, U U
AU  - Karniychuk UU
AD  - Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 
      Merelbeke, Belgium. uladzimir.karniychuk@UGent.be
FAU - De Spiegelaere, W
AU  - De Spiegelaere W
FAU - Nauwynck, H J
AU  - Nauwynck HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130523
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (CD8 Antigens)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
SB  - IM
MH  - Animals
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - CD8 Antigens/*analysis
MH  - Disease Models, Animal
MH  - Endometrium/*immunology/pathology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Profiling
MH  - Killer Cells, Natural/chemistry/*immunology
MH  - Macrophages/chemistry/*immunology
MH  - Placenta/*immunology/pathology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/pathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Pregnancy
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, Cell Surface/analysis
MH  - Sialic Acid Binding Ig-like Lectin 1/*analysis
MH  - Swine
OTO - NOTNLM
OT  - CD163
OT  - Endometrium
OT  - NK cells
OT  - PRRSV
OT  - Placenta
OT  - Sialoadhesin
EDAT- 2013/05/28 06:00
MHDA- 2014/03/01 06:00
CRDT- 2013/05/28 06:00
PHST- 2013/02/02 00:00 [received]
PHST- 2013/05/03 00:00 [revised]
PHST- 2013/05/06 00:00 [accepted]
PHST- 2013/05/28 06:00 [entrez]
PHST- 2013/05/28 06:00 [pubmed]
PHST- 2014/03/01 06:00 [medline]
AID - S0168-1702(13)00170-6 [pii]
AID - 10.1016/j.virusres.2013.05.005 [doi]
PST - ppublish
SO  - Virus Res. 2013 Sep;176(1-2):285-91. doi: 10.1016/j.virusres.2013.05.005. Epub 
      2013 May 23.