PMID- 23707907
OWN - NLM
STAT- MEDLINE
DCOM- 20130827
LR  - 20171116
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1828
IP  - 9
DP  - 2013 Sep
TI  - Evaluation of membrane models and their composition for islet amyloid 
      polypeptide-membrane aggregation.
PG  - 2091-8
LID - S0005-2736(13)00160-0 [pii]
LID - 10.1016/j.bbamem.2013.05.014 [doi]
AB  - Human islet amyloid polypeptide (IAPP) forms amyloid fibrils in the pancreatic 
      islets of patients suffering from type 2 diabetes mellitus (T2DM). The formation 
      of IAPP fibrils has been shown to cause membrane damage which most likely is 
      responsible for the death of pancreatic islet beta-cells during the pathogenesis of 
      T2DM. Several studies have demonstrated a clear interaction between IAPP and 
      lipid membranes. However the effect of different lipid compositions and of 
      various membrane mimetics (including micelles, bicelles, SUV and LUV) on fibril 
      formation kinetics and fibril morphology has not yet systematically been 
      analysed. Here we report that the interaction of IAPP with various membrane 
      models promoted different processes of fibril formation. Our data reveal that in 
      SDS and DPC micelles, IAPP adopts a stable alpha-helical structure for several days, 
      suggesting that the micelle models may stabilize monomeric or small oligomeric 
      species of IAPP. In contrast, zwitterionic DMPC/DHPC bicelles and DOPC SUV 
      accelerate the fibril formation compared to zwitterionic DOPC LUV, indicating 
      that the size of the membrane model and its curvature influence the fibrillation 
      process. Negatively charged membranes decrease the lag-time of the fibril 
      formation kinetics while phosphatidylethanolamine and cholesterol have an 
      opposite effect, probably due to the modulation of the physical properties of the 
      membrane and/or due to direct interactions with IAPP within the membrane core. 
      Finally, our results show that the modulation of lipid composition influences not 
      only the growth of fibrils at the membrane surface but also the interactions of 
      beta-sheet oligomers with membranes.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Caillon, Lucie
AU  - Caillon L
AD  - UPMC Univ Paris 06, UMR 7203 CNRS-UPMC-ENS, Laboratoire des Biomolecules, 4 place 
      Jussieu, 75005 Paris, France.
FAU - Lequin, Olivier
AU  - Lequin O
FAU - Khemtemourian, Lucie
AU  - Khemtemourian L
LA  - eng
PT  - Journal Article
DEP - 20130523
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (1,2-dihexadecyl-sn-glycero-3-phosphocholine)
RN  - 0 (Islet Amyloid Polypeptide)
RN  - 0 (Lipid Bilayers)
RN  - 0 (Micelles)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Phosphatidylethanolamines)
RN  - 0 (Phospholipid Ethers)
RN  - 0 (Unilamellar Liposomes)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EDS2L3ODLV (1,2-oleoylphosphatidylcholine)
RN  - U86ZGC74V5 (Dimyristoylphosphatidylcholine)
SB  - IM
MH  - Cholesterol/*chemistry
MH  - Dimyristoylphosphatidylcholine/*chemistry
MH  - Humans
MH  - Islet Amyloid Polypeptide/*chemistry/ultrastructure
MH  - Lipid Bilayers/*chemistry
MH  - Micelles
MH  - Microscopy, Electron
MH  - Models, Biological
MH  - Phosphatidylcholines/*chemistry
MH  - Phosphatidylethanolamines/*chemistry
MH  - Phospholipid Ethers/*chemistry
MH  - Protein Structure, Secondary
MH  - Static Electricity
MH  - Unilamellar Liposomes/chemistry
OTO - NOTNLM
OT  - 1,1,1,3,3,3-hexafluoro-2-propanol
OT  - 1,2-dihexanoyl-sn-glycero-3-phosphocholine
OT  - 1,2-dimyristoyl-sn-glycero-3-phosphocholine
OT  - 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
OT  - 1,2-dioleoyl-sn-glycero-3-phospho-L-serine
OT  - 1,2-dioleoyl-sn-glycero-3-phosphocholine
OT  - 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
OT  - 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
OT  - 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
OT  - Aggregation kinetics
OT  - Amyloid
OT  - CD
OT  - Chol
OT  - DHPC
OT  - DMPC
OT  - DMSO
OT  - DOPC
OT  - DOPE
OT  - DOPG
OT  - DOPS
OT  - DPC
OT  - DPPC
OT  - Fibril morphology
OT  - HFIP
OT  - IAPP
OT  - LUV
OT  - Model membranes (LUV SUV, bicelles, micelles)
OT  - POPC
OT  - Phospholipid
OT  - SDS
OT  - SM
OT  - ThT
OT  - Thioflavin T
OT  - cholesterol
OT  - circular dichroism
OT  - dimethyl sulfoxide
OT  - dodecyl phosphocholine
OT  - human Islet Amyloid Polypeptide
OT  - large unilamellar vesicle
OT  - sodium dodecyl sulphate
OT  - sphingomyelin
EDAT- 2013/05/28 06:00
MHDA- 2013/08/28 06:00
CRDT- 2013/05/28 06:00
PHST- 2012/10/31 00:00 [received]
PHST- 2013/04/26 00:00 [revised]
PHST- 2013/05/13 00:00 [accepted]
PHST- 2013/05/28 06:00 [entrez]
PHST- 2013/05/28 06:00 [pubmed]
PHST- 2013/08/28 06:00 [medline]
AID - S0005-2736(13)00160-0 [pii]
AID - 10.1016/j.bbamem.2013.05.014 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2013 Sep;1828(9):2091-8. doi: 10.1016/j.bbamem.2013.05.014. 
      Epub 2013 May 23.