PMID- 23708751
OWN - NLM
STAT- MEDLINE
DCOM- 20140102
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 48
DP  - 2013 Oct 15
TI  - Image-based ELISA on an activated polypropylene microtest plate--a 
      spectrophotometer-free low cost assay technique.
PG  - 287-92
LID - S0956-5663(13)00286-8 [pii]
LID - 10.1016/j.bios.2013.04.020 [doi]
AB  - In this communication, we report ELISA technique on an activated polypropylene 
      microtest plate (APPmicroTP) as an illustrative example of a low cost diagnostic 
      assay. Activated test zone in APPmicroTP binds a capture biomolecule through covalent 
      linkage thereby, eliminating non-specific binding often prevalent in absorption 
      based techniques. Efficacy of APPmicroTP is demonstrated by detecting human 
      immunoglobulin G (IgG), human immunoglobulin E (IgE) and Aspergillus fumigatus 
      antibody in patient's sera. Detection is done by taking the image of the assay 
      solution by a desktop scanner and analyzing the color of the image. Human IgE 
      quantification by color saturation in the image-based assay shows excellent 
      correlation with absorbance-based assay (Pearson correlation coefficient, 
      r=0.992). Significance of the relationship is seen from its p value which is 
      4.087e-11. Performance of APPmicroTP is also checked with respect to microtiter plate 
      and paper-based ELISA. APPmicroTP can quantify an analyte as precisely as in 
      microtiter plate with insignificant non-specific binding, a necessary 
      prerequisite for ELISA assay. In contrast, paper-ELISA shows high non-specific 
      binding in control sera (false positive). Finally, we have carried out ELISA 
      steps on APPmicroTP by ultrasound waves on a sonicator bath and the results show that 
      even in 8 min, it can convincingly differentiate a test sample from a control 
      sample. In short, spectrophotometer-free image-based miniaturized ELISA on APPmicroTP 
      is precise, reliable, rapid, and sensitive and could be a good substitute for 
      conventional immunoassay procedures widely used in clinical and research 
      laboratories.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Parween, Shahila
AU  - Parween S
AD  - Department of Chemical and Systems Biology, CSIR-Institute of Genomics and 
      Integrative Biology, Mall Road, Delhi 110007, India.
FAU - Nahar, Pradip
AU  - Nahar P
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130430
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Polypropylenes)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
CIN - Biosens Bioelectron. 2015 Jan 15;63:605-8. PMID: 24975615
CIN - Biosens Bioelectron. 2015 Jan 15;63:602-4. PMID: 24994570
MH  - Antibodies, Fungal/*blood
MH  - Aspergillosis/*blood/immunology
MH  - Aspergillus fumigatus/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/economics/*instrumentation
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Immunoglobulin G/*analysis
MH  - Polypropylenes/chemistry
MH  - Sensitivity and Specificity
MH  - Time Factors
EDAT- 2013/05/28 06:00
MHDA- 2014/01/03 06:00
CRDT- 2013/05/28 06:00
PHST- 2013/02/04 00:00 [received]
PHST- 2013/04/05 00:00 [revised]
PHST- 2013/04/18 00:00 [accepted]
PHST- 2013/05/28 06:00 [entrez]
PHST- 2013/05/28 06:00 [pubmed]
PHST- 2014/01/03 06:00 [medline]
AID - S0956-5663(13)00286-8 [pii]
AID - 10.1016/j.bios.2013.04.020 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2013 Oct 15;48:287-92. doi: 10.1016/j.bios.2013.04.020. Epub 
      2013 Apr 30.