PMID- 23710460
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20240324
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2013
DP  - 2013
TI  - Late adherent human bone marrow stromal cells form bone and restore the 
      hematopoietic microenvironment in vivo.
PG  - 790842
LID - 10.1155/2013/790842 [doi]
LID - 790842
AB  - Bone marrow stromal cells (BMSCs) are a valuable resource for skeletal 
      regenerative medicine because of their osteogenic potential. In spite of the very 
      general term "stem cell," this population of cells is far from homogeneous, and 
      different BMSCs clones have greatly different phenotypic properties and, 
      therefore, potentially different therapeutic potential. Adherence to a culture 
      flask surface is a primary defining characteristic of BMSCs. We hypothesized that 
      based on the adherence time we could obtain an enriched population of cells with 
      a greater therapeutic potential. We characterized two populations of bone 
      marrow-derived cells, those that adhered by three days (R-cells) and those that 
      did not adhere by three days but did by six days (L-cells). Clones derived from 
      L-cells could be induced into adipogenic, chondrogenic, and osteogenic 
      differentiation in vitro. L-cells appeared to have greater proliferative 
      capacity, as manifested by larger colony diameter and clones with higher CD146 
      expression. Only clones from L-cells developed bone marrow stroma in vivo. We 
      conclude that the use of late adherence of BMSCs is one parameter that can be 
      used to enrich for cells that will constitute a superior final product for cell 
      therapy in orthopedics.
FAU - Vianna, Veronica Fernandes
AU  - Vianna VF
AD  - Clinical and Basic Research Division, National Institute of Traumatology and 
      Orthopaedics, Avenida Brasil 500, 20940-070 Rio de Janeiro, RJ, Brazil. 
      vvianna@into.saude.gov.br
FAU - Bonfim, Danielle Cabral
AU  - Bonfim DC
FAU - Cavalcanti, Amanda Dos Santos
AU  - Cavalcanti Ados S
FAU - Fernandes, Marco Cury
AU  - Fernandes MC
FAU - Kahn, Suzana Assad
AU  - Kahn SA
FAU - Casado, Priscila Ladeira
AU  - Casado PL
FAU - Lima, Inaya Correa
AU  - Lima IC
FAU - Murray, Samuel S
AU  - Murray SS
FAU - Murray, Elsa J Brochmann
AU  - Murray EJ
FAU - Duarte, Maria Eugenia Leite
AU  - Duarte ME
LA  - eng
PT  - Journal Article
DEP - 20130424
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (CD146 Antigen)
RN  - 0 (MCAM protein, human)
SB  - IM
MH  - Adult
MH  - CD146 Antigen/biosynthesis
MH  - Cell Adhesion/*genetics
MH  - *Cell Differentiation
MH  - Cell Lineage/genetics
MH  - Cells, Cultured
MH  - Female
MH  - Fibroblasts/cytology
MH  - Gene Expression Regulation
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology
MH  - Middle Aged
MH  - *Osteogenesis
MH  - Regenerative Medicine
MH  - *Stem Cell Niche
PMC - PMC3655461
EDAT- 2013/05/28 06:00
MHDA- 2014/01/01 06:00
PMCR- 2013/04/24
CRDT- 2013/05/28 06:00
PHST- 2012/11/30 00:00 [received]
PHST- 2013/02/23 00:00 [revised]
PHST- 2013/03/14 00:00 [accepted]
PHST- 2013/05/28 06:00 [entrez]
PHST- 2013/05/28 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
PHST- 2013/04/24 00:00 [pmc-release]
AID - 10.1155/2013/790842 [doi]
PST - ppublish
SO  - Biomed Res Int. 2013;2013:790842. doi: 10.1155/2013/790842. Epub 2013 Apr 24.