PMID- 23711388
OWN - NLM
STAT- MEDLINE
DCOM- 20130828
LR  - 20211203
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 56
IP  - 1-2
DP  - 2013 Nov
TI  - Proteomic changes induced by histone demethylase JMJD3 in TNF alpha-treated human 
      monocytic (THP-1) cells.
PG  - 113-22
LID - S0161-5890(13)00164-8 [pii]
LID - 10.1016/j.molimm.2013.04.013 [doi]
AB  - JMJD3, a Jumonji C family histone demethylase, plays an important role in the 
      regulation of inflammation induced by the transcription factor nuclear 
      factor-kappa B (NF-kappaB) in response to various stimuli. JMJD3 is a histone-3 
      lysine-27 trimethylation (H3K27me3) demethylase, a histone mark associated with 
      transcriptional repression and activation of a diverse set of genes. The present 
      study assessed stable JMJD3 knockdown (KD)-dependent proteomic profiling in human 
      leukemia monocyte (THP-1) cells to analyze the JMJD3-mediated differential 
      changes of marker expression in inflammatory cells. To analyze the protein 
      expression profile of tumor necrosis factor-alpha (TNF-alpha)-stimulated JMJD3-kd 
      THP-1 cells, we employed 
      matrix-assisted-laser-desorption/ionization-time-of-flight mass spectrometry 
      (MALDI-TOF MS). Additionally, Ingenuity Pathways Analysis (IPA) was applied to 
      establish the molecular networks. A comparative proteomic profile was determined 
      in TNF-alpha-treated both of JMJD3-kd THP-1 cells and THP-1 scrambled (sc) cells. The 
      expression of tripartite motif protein (TRIM5), glutathione peroxidase (GPx), 
      glia maturation factor-gamma (GMFG), caspase recruitment domain family, member 14 
      (CARMA2), and dUTP pyrophosphatase were significantly down-regulated, whereas 
      heat shock protein beta-1 (HspB1) and prohibition were significantly up-regulated 
      in JMJD3-kd THP-1 cells. The molecular and signaling networks of the 
      differentially expressed proteins in JMJD3-kd THP-1 cells were determined by IPA. 
      The molecular network signatures and functional proteomics obtained in this study 
      may facilitate the suppression of different key inflammatory regulators through 
      JMJD3-attenuation, which would be crucial to evaluate potential therapeutic 
      targets and to elucidate the molecular mechanism of JMJD3-kd dependent effects in 
      THP-1 cells.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Das, Amitabh
AU  - Das A
AD  - Department of Molecular & Life Science, Hanyang University, Ansan, Republic of 
      Korea. amitabhdas.kn@gmail.com
FAU - Das, Nando Dulal
AU  - Das ND
FAU - Jung, Kyoung Hwa
AU  - Jung KH
FAU - Park, Ji Hyun
AU  - Park JH
FAU - Lee, Hyung Tae
AU  - Lee HT
FAU - Han, DalMuri
AU  - Han D
FAU - Choi, Mi Ran
AU  - Choi MR
FAU - Kang, Sung Chul
AU  - Kang SC
FAU - Chai, Young Gyu
AU  - Chai YG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130525
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Antiviral Restriction Factors)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (NUDC protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteome)
RN  - 0 (Tripartite Motif Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.11.- (Jumonji Domain-Containing Histone Demethylases)
RN  - EC 1.14.11.- (KDM6B protein, human)
RN  - EC 2.3.2.27 (TRIM5 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Antiviral Restriction Factors
MH  - Blotting, Western
MH  - Carrier Proteins/genetics/metabolism
MH  - Cell Cycle Proteins/genetics/metabolism
MH  - Cell Line, Tumor
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Jumonji Domain-Containing Histone Demethylases/genetics/*metabolism
MH  - Monocytes/*drug effects/metabolism/pathology
MH  - Nuclear Proteins/genetics/metabolism
MH  - Proteome/genetics/metabolism
MH  - Proteomics/*methods
MH  - RNA Interference
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/drug effects
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Tripartite Motif Proteins
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Ubiquitin-Protein Ligases
EDAT- 2013/05/29 06:00
MHDA- 2013/08/29 06:00
CRDT- 2013/05/29 06:00
PHST- 2012/10/05 00:00 [received]
PHST- 2013/03/16 00:00 [revised]
PHST- 2013/04/23 00:00 [accepted]
PHST- 2013/05/29 06:00 [entrez]
PHST- 2013/05/29 06:00 [pubmed]
PHST- 2013/08/29 06:00 [medline]
AID - S0161-5890(13)00164-8 [pii]
AID - 10.1016/j.molimm.2013.04.013 [doi]
PST - ppublish
SO  - Mol Immunol. 2013 Nov;56(1-2):113-22. doi: 10.1016/j.molimm.2013.04.013. Epub 
      2013 May 25.