PMID- 23712875
OWN - NLM
STAT- MEDLINE
DCOM- 20140923
LR  - 20140103
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Linking)
VI  - 68
IP  - 1
DP  - 2014 Jan
TI  - Comparative study of different latent infections of herpes simplex virus type I 
      in a murine model.
PG  - 159-62
LID - 10.1007/s12013-013-9682-9 [doi]
AB  - This study aims to compare the different latent infections of herpes simplex 
      virus type I in a murine model. One hundred and twenty BALB/c mice were randomly 
      assigned into either of three groups: intravenous inoculation group, ocular 
      abrasion group, and intranasal inoculation group. Six weeks later, the trigeminal 
      ganglia (TG) were removed to detect the expression of HSV-I antigen. HSV DNA in 
      TG was also detected by polymerase chain reaction to confirm latent infection. 
      The rate of HSV DNA in TG detected in the intravenous inoculation group was 18/22 
      and 22/26 in the ocular abrasion group, both of which were higher than the rate 
      detected in the intranasal inoculation group (18/30). The expression of HSV 
      antigen in TG in these three groups was all negative. Mortality rate in the 
      intravenous inoculation group was 8/30, which was much higher than those of the 
      two other groups. Intranasal virus dripping, cornea abrasion, and intravenous 
      injection can detect latent HSV-I infection in a murine model. Compared to two 
      other groups, the cornea abrasion group showed less severe signs, a quicker 
      recovery rate in acute infection, and higher incidence rate of latent infection. 
      Therefore, it is an ideal method in the presence of latent HSV-I infection.
FAU - Huang, Wen
AU  - Huang W
AD  - Department of Neurology, Xinqiao Hospital, Third Military Medical University, 
      Chongqing, 400037, People's Republic of China.
FAU - Zhao, Ping
AU  - Zhao P
FAU - Chen, Xiao
AU  - Chen X
FAU - Li, Ping
AU  - Li P
FAU - Zhao, Gaonian
AU  - Zhao G
FAU - Xu, Mingming
AU  - Xu M
FAU - Chen, Xiuying
AU  - Chen X
FAU - Xie, Peng
AU  - Xie P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Cornea/virology
MH  - DNA, Viral/analysis
MH  - Disease Models, Animal
MH  - Herpes Simplex/pathology/*virology
MH  - Herpesvirus 1, Human/genetics/*physiology
MH  - Injections, Intravenous
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Polymerase Chain Reaction
MH  - Severity of Illness Index
MH  - Trigeminal Ganglion/virology
MH  - *Virus Latency
EDAT- 2013/05/29 06:00
MHDA- 2014/09/24 06:00
CRDT- 2013/05/29 06:00
PHST- 2013/05/29 06:00 [entrez]
PHST- 2013/05/29 06:00 [pubmed]
PHST- 2014/09/24 06:00 [medline]
AID - 10.1007/s12013-013-9682-9 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2014 Jan;68(1):159-62. doi: 10.1007/s12013-013-9682-9.