PMID- 23715170
OWN - NLM
STAT- MEDLINE
DCOM- 20131021
LR  - 20211021
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 19
DP  - 2013 May 27
TI  - Effect of common single-nucleotide polymorphisms in acetylsalicylic acid 
      metabolic pathway genes on platelet reactivity in patients with diabetes.
PG  - 394-408
LID - 10.12659/MSM.883922 [doi]
AB  - BACKGROUND: Platelet reactivity in patients on acetylsalicylic acid (ASA) therapy 
      can be influenced by physiological or pathological conditions affecting ASA 
      pharmacokinetics or pharmacodynamics. The mechanism of such variability in the 
      therapeutic response to ASA, particularly in diabetic patients, is poorly 
      understood. The rate of elimination of ASA and its metabolite, salicylic acid 
      (SA), is likely a major factor determining drug efficacy. The objective of this 
      study was to investigate the effect of genetic polymorphisms in the selected 
      candidate genes within the ASA metabolic pathway on the platelet reactivity and 
      concentration of ASA and thromboxane A(2) (TxA(2)) metabolites in a population of 
      patients with type 2 diabetes mellitus (T2DM). MATERIAL AND METHODS: The study 
      cohort consisted of 287 Caucasians with T2DM who had been taking ASA tablets at 
      the dose of 75 mg per day for at least 3 months. Platelet reactivity analyses 
      were performed using VerifyNow Aspirin and PFA-100 assays. The measured ASA 
      metabolite included salicylic acid (ASA), and TxA(2) metabolites included serum 
      TxB(2) and urinary 11-dh-TxB(2). Genotyping for the selected 18 single-nucleotide 
      polymorphisms (SNPs) within 5 genes of the ASA metabolic pathway was performed 
      using a Sequenom iPLEX platform. RESULTS: No statistically significant 
      association was observed between the investigated SNPs genotypes, platelet 
      reactivity, and measured metabolites in the investigated cohort of patients. 
      CONCLUSIONS: The results of our study failed to confirm that the selected 
      variants in the genes within the ASA metabolic pathway might contribute to 
      platelet reactivity in a diabetic population treated with ASA.
FAU - Postula, Marek
AU  - Postula M
AD  - Department of Cardiology, Medical University of Warsaw, Warsaw, Poland.
FAU - Janicki, Piotr K
AU  - Janicki PK
FAU - Rosiak, Marek
AU  - Rosiak M
FAU - Kaplon-Cieslicka, Agnieszka
AU  - Kaplon-Cieslicka A
FAU - Kondracka, Agnieszka
AU  - Kondracka A
FAU - Trzepla, Ewa
AU  - Trzepla E
FAU - Filipiak, Krzysztof J
AU  - Filipiak KJ
FAU - Kosior, Dariusz A
AU  - Kosior DA
FAU - Czlonkowski, Andrzej
AU  - Czlonkowski A
FAU - Opolski, Grzegorz
AU  - Opolski G
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20130527
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - R16CO5Y76E (Aspirin)
SB  - IM
MH  - Aged
MH  - Aspirin/*metabolism
MH  - Blood Platelets/*metabolism
MH  - Demography
MH  - Diabetes Mellitus, Type 2/*genetics/*metabolism
MH  - Female
MH  - Gene Frequency/genetics
MH  - Genotype
MH  - Humans
MH  - Male
MH  - Metabolic Networks and Pathways/*genetics
MH  - Polymorphism, Single Nucleotide/*genetics
PMC - PMC3670858
EDAT- 2013/05/30 06:00
MHDA- 2013/10/22 06:00
PMCR- 2013/05/27
CRDT- 2013/05/30 06:00
PHST- 2013/05/30 06:00 [entrez]
PHST- 2013/05/30 06:00 [pubmed]
PHST- 2013/10/22 06:00 [medline]
PHST- 2013/05/27 00:00 [pmc-release]
AID - 883922 [pii]
AID - 10.12659/MSM.883922 [doi]
PST - epublish
SO  - Med Sci Monit. 2013 May 27;19:394-408. doi: 10.12659/MSM.883922.