PMID- 23717008
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130530
LR  - 20211021
IS  - 1013-9087 (Print)
IS  - 2005-3894 (Electronic)
IS  - 1013-9087 (Linking)
VI  - 25
IP  - 2
DP  - 2013 May
TI  - Potential Immunoinflammatory Role of Staphylococcal Enterotoxin A in Atopic 
      Dermatitis: Immunohistopathological Analysis and in vitro Assay.
PG  - 173-80
LID - 10.5021/ad.2013.25.2.173 [doi]
AB  - BACKGROUND: The underlying mechanism of atopic dermatitis (AD) exacerbated by 
      Staphylococcus aureus has not been established. However, we demonstrated recently 
      that the majority of S. aureus strains colonized in the skin of Korean AD 
      patients carried genes encoding staphylococcal enterotoxin A (SEA) and/or toxic 
      shock syndrome toxin-1 (TSST-1). OBJECTIVE: To clarify the role of staphylococcal 
      superantigen, SEA in AD. METHODS: With the lesional skin of 9 AD patients and 
      normal looking skin of one healthy adult, we examined first the expression of 
      SEA, staphylococcal enterotoxin B (SEB), and TSST-1 using immunohistochemical 
      analysis. In addition, we investigated the effects of SEA on the expression of 
      inflammation-related adhesion molecules and cytokines in human HaCaT 
      keratinocytes and Human Umbilical Vein Endothelial Cells (HUVECs) by reverse 
      transcriptase-polymerase chain reaction (RT-PCR) analysis and enzyme-linked 
      immunosorbent assay. RESULTS: Staphylococcal protein A (SPA) and SEA were 
      detected with increased immunoreactivity in AD patients. However, TSST-1 showed 
      mild-to-moderate immunoreactivity in AD patients, whereas SEB was minimally 
      detected. In the double immunofluorescence investigation, SEA and SPA were well 
      co-localized. SEA induced upregulation of adhesion molecules and elicited 
      inflammatory responses in HaCaT keratinocytes and HUVECs. CONCLUSION: This study 
      demonstrates the importance of SEA as an immunoinflammatory triggering factor of 
      AD in Koreans.
FAU - Lee, Hee-Woo
AU  - Lee HW
AD  - Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul 
      National University, Seoul, Korea.
FAU - Kim, Sung Min
AU  - Kim SM
FAU - Kim, Jung Min
AU  - Kim JM
FAU - Oh, Byung Min
AU  - Oh BM
FAU - Kim, Jun Young
AU  - Kim JY
FAU - Jung, Han Jin
AU  - Jung HJ
FAU - Lim, Hyun Jung
AU  - Lim HJ
FAU - Kim, Byung Soo
AU  - Kim BS
FAU - Lee, Weon Ju
AU  - Lee WJ
FAU - Lee, Seok-Jong
AU  - Lee SJ
FAU - Kim, Do Won
AU  - Kim DW
LA  - eng
PT  - Journal Article
DEP - 20130510
PL  - Korea (South)
TA  - Ann Dermatol
JT  - Annals of dermatology
JID - 8916577
PMC - PMC3662910
OTO - NOTNLM
OT  - Atopic dermatitis
OT  - Staphylococcal enterotoxin A
OT  - Staphylococcus aureus
EDAT- 2013/05/30 06:00
MHDA- 2013/05/30 06:01
PMCR- 2013/05/01
CRDT- 2013/05/30 06:00
PHST- 2011/10/24 00:00 [received]
PHST- 2012/01/26 00:00 [revised]
PHST- 2012/02/21 00:00 [accepted]
PHST- 2013/05/30 06:00 [entrez]
PHST- 2013/05/30 06:00 [pubmed]
PHST- 2013/05/30 06:01 [medline]
PHST- 2013/05/01 00:00 [pmc-release]
AID - 10.5021/ad.2013.25.2.173 [doi]
PST - ppublish
SO  - Ann Dermatol. 2013 May;25(2):173-80. doi: 10.5021/ad.2013.25.2.173. Epub 2013 May 
      10.