PMID- 23719635
OWN - NLM
STAT- MEDLINE
DCOM- 20140221
LR  - 20190720
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 36
IP  - 8
DP  - 2013
TI  - Manassantin B isolated from Saururus chinensis inhibits 
      cyclooxygenase-2-dependent prostaglandin D2 generation by blocking Fyn-mediated 
      nuclear factor-kappaB and mitogen activated protein kinase pathways in bone 
      marrow derived-mast cells.
PG  - 1370-4
AB  - The authors investigated the effect of manassantin B (Man B) isolated from 
      Saururus chinensis (S. chinensis) on cyclooxygenase-2 (COX-2)-dependent 
      prostaglandin D2 (PGD2) generation in mouse bone marrow derived-mast cells 
      (BMMCs). Man B inhibited the generation of PGD2 dose-dependently by inhibiting 
      COX-2 expression in immunoglobulin E (IgE)/Ag-stimulated BMMCs. To elucidate the 
      mechanism responsible for the inhibition of COX-2 expression by Man B, the 
      effects of Man B on the activation of nuclear factor-kappaB (NF-kappaB), a 
      transcription factor essential and mitogen-activated protein kinases (MAPKs) for 
      COX-2 induction, were examined. Man B attenuated the nuclear translocation of 
      NF-kappaB p65 and its DNA-binding activity by inhibiting inhibitors of kappa Balpha 
      (IkappaBalpha) degradation and concomitantly suppressing IkappaB kinase (IKK) 
      phosphorylation. In addition, Man B suppressed phosphorylation of MAPKs including 
      extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun NH2-terminal kinase 
      (JNK) and p38. It was also found that Man B suppressed Fyn kinase activation and 
      consequent downstream signaling processes, including those involving Syk, Gab2, 
      and Akt. Taken together, the present results suggest that Man B suppresses COX-2 
      dependent PGD2 generation by primarily inhibiting Fyn kinase in FcepsilonRI-mediated 
      mast cells.
FAU - Lu, Yue
AU  - Lu Y
AD  - College of Pharmacy, Yeungnam University; Gyeongsan 712-749, Republic of Korea.
FAU - Hwang, Seung-Lark
AU  - Hwang SL
FAU - Son, Jong Keun
AU  - Son JK
FAU - Chang, Hyeun Wook
AU  - Chang HW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130528
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Cyclooxygenase 2 Inhibitors)
RN  - 0 (Furans)
RN  - 0 (NF-kappa B)
RN  - 0 (manassantin B)
RN  - EC 1.14.99.- (Ptgs2 protein, mouse)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 2.7.10.2 (Fyn protein, mouse)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-fyn)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - RXY07S6CZ2 (Prostaglandin D2)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Cells, Cultured
MH  - Cyclooxygenase 2/metabolism
MH  - Cyclooxygenase 2 Inhibitors/isolation & purification/*pharmacology
MH  - Furans/isolation & purification/*pharmacology
MH  - Male
MH  - Mast Cells/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - NF-kappa B/metabolism
MH  - Phytotherapy
MH  - Plant Roots/chemistry
MH  - Prostaglandin D2/*antagonists & inhibitors/metabolism
MH  - Proto-Oncogene Proteins c-fyn/metabolism
MH  - Saururaceae
EDAT- 2013/05/31 06:00
MHDA- 2014/02/22 06:00
CRDT- 2013/05/31 06:00
PHST- 2013/05/31 06:00 [entrez]
PHST- 2013/05/31 06:00 [pubmed]
PHST- 2014/02/22 06:00 [medline]
AID - DN/JST.JSTAGE/bpb/b13-00146 [pii]
AID - 10.1248/bpb.b13-00146 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2013;36(8):1370-4. doi: 10.1248/bpb.b13-00146. Epub 2013 May 28.