PMID- 23719940
OWN - NLM
STAT- MEDLINE
DCOM- 20131216
LR  - 20170420
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1024
DP  - 2013
TI  - Methods of analysis of dendritic cell-derived exosome-shuttle microRNA and its 
      horizontal propagation between dendritic cells.
PG  - 19-40
LID - 10.1007/978-1-62703-453-1_3 [doi]
AB  - Exosomes are extremely small (<100 nm) membrane vesicles, generated in the 
      endocytic compartment that are released to the extracellular milieu by living 
      cells. Although the biological function of exosomes in vivo remains unclear, they 
      seem to function as mechanisms of cell-to-cell communication for horizontal 
      transfer of proteins, antigens, prions, morphogens, mRNA, and noncoding 
      regulatory RNAs, including microRNAs (miRNAs) (also known as exosome-shuttle 
      miRNAs). Dendritic cells (DCs), the most potent professional antigen-presenting 
      leukocytes of the immune system, release relatively high levels of exosomes and 
      also interact with free exosomes present in the extracellular space. Therefore, 
      DCs constitute a good model for the analysis of exosome-shuttle miRNAs and their 
      horizontal propagation between cells. This chapter provides basic protocols for 
      purification of exosomes released by mouse bone marrow-derived DCs, analysis of 
      their miRNA content, and assessment of the function of exosome-shuttle miRNAs, 
      once they are transferred to target/acceptor DCs.
FAU - Montecalvo, Angela
AU  - Montecalvo A
AD  - Thomas E. Starzl Transplantation Institute, Department of Surgery, University of 
      Pittsburgh Medical Center, Pittsburgh, PA, USA.
FAU - Larregina, Adriana T
AU  - Larregina AT
FAU - Morelli, Adrian E
AU  - Morelli AE
LA  - eng
GR  - R01 AI077511/AI/NIAID NIH HHS/United States
GR  - T32 A1089433/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*chemistry/cytology/metabolism
MH  - Cell Communication
MH  - Culture Media, Conditioned/chemistry
MH  - Dendritic Cells/*chemistry/cytology/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Exosomes/*chemistry/genetics/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*isolation & purification
MH  - Microscopy, Electron
MH  - RNA Transport
MH  - Ultracentrifugation
EDAT- 2013/05/31 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/05/31 06:00
PHST- 2013/05/31 06:00 [entrez]
PHST- 2013/05/31 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
AID - 10.1007/978-1-62703-453-1_3 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;1024:19-40. doi: 10.1007/978-1-62703-453-1_3.