PMID- 23720369
OWN - NLM
STAT- MEDLINE
DCOM- 20140213
LR  - 20220409
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 20
IP  - 8
DP  - 2013 Aug
TI  - Human monocyte-derived dendritic cells exposed to microorganisms involved in 
      hypersensitivity pneumonitis induce a Th1-polarized immune response.
PG  - 1133-42
LID - 10.1128/CVI.00043-13 [doi]
AB  - Hypersensitivity pneumonitis (HP) is an immunoallergic disease characterized by a 
      prominent interstitial infiltrate composed predominantly of lymphocytes secreting 
      inflammatory cytokines. Dendritic cells (DCs) are known to play a pivotal role in 
      the lymphocytic response. However, their cross talk with microorganisms that 
      cause HP has yet to be elucidated. This study aimed to investigate the initial 
      interactions between human monocyte-derived DCs (MoDCs) and four microorganisms 
      that are different in nature (Saccharopolyspora rectivirgula [actinomycetes], 
      Mycobacterium immunogenum [mycobacteria], and Wallemia sebi and Eurotium 
      amstelodami [filamentous fungi]) and are involved in HP. Our objectives were to 
      determine the cross talk between MoDCs and HP-causative agents and to determine 
      whether the resulting immune response varied according to the microbial extract 
      tested. The phenotypic activation of MoDCs was measured by the increased 
      expression of costimulatory molecules and levels of cytokines in supernatants. 
      The functional activation of MoDCs was measured by the ability of MoDCs to induce 
      lymphocytic proliferation and differentiation in a mixed lymphocytic reaction 
      (MLR). E. amstelodami-exposed (EA) MoDCs expressed higher percentages of 
      costimulatory molecules than did W. sebi-exposed (WS), S. rectivirgula-exposed 
      (SR), or M. immunogenum-exposed (MI) MoDCs (P < 0.05, Wilcoxon signed-rank test). 
      EA-MoDCs, WS-MoDCs, SR-MoDCs, and MI-MoDCs induced CD4(+) T cell proliferation 
      and a Th1-polarized immune response. The present study provides evidence that, 
      although differences were initially observed between MoDCs exposed to filamentous 
      fungi and MoDCs exposed to bacteria, a Th1 response was ultimately promoted by 
      DCs regardless of the microbial extract tested.
FAU - Bellanger, Anne-Pauline
AU  - Bellanger AP
AD  - University of Franche-Comte, Besancon, France. apbellanger@chu-besancon.fr
FAU - Pallandre, Jean-Rene
AU  - Pallandre JR
FAU - Borg, Christophe
AU  - Borg C
FAU - Loeffert, Sophie
AU  - Loeffert S
FAU - Gbaguidi-Haore, Houssein
AU  - Gbaguidi-Haore H
FAU - Millon, Laurence
AU  - Millon L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130529
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Cytokines)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Alveolitis, Extrinsic Allergic/*immunology/*microbiology
MH  - Bacteria/*immunology
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology
MH  - Fungi/*immunology
MH  - Humans
MH  - Receptors, Immunologic/biosynthesis
MH  - Th1 Cells/*immunology
PMC - PMC3754498
EDAT- 2013/05/31 06:00
MHDA- 2014/02/14 06:00
PMCR- 2014/02/01
CRDT- 2013/05/31 06:00
PHST- 2013/05/31 06:00 [entrez]
PHST- 2013/05/31 06:00 [pubmed]
PHST- 2014/02/14 06:00 [medline]
PHST- 2014/02/01 00:00 [pmc-release]
AID - CVI.00043-13 [pii]
AID - 00043-13 [pii]
AID - 10.1128/CVI.00043-13 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2013 Aug;20(8):1133-42. doi: 10.1128/CVI.00043-13. Epub 
      2013 May 29.