PMID- 23720728
OWN - NLM
STAT- MEDLINE
DCOM- 20130918
LR  - 20211021
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 87
IP  - 15
DP  - 2013 Aug
TI  - Constitutive interferon-inducible protein 16-inflammasome activation during 
      Epstein-Barr virus latency I, II, and III in B and epithelial cells.
PG  - 8606-23
LID - 10.1128/JVI.00805-13 [doi]
AB  - Epstein-Barr virus (EBV), etiologically linked with human B-cell malignancies and 
      nasopharyngeal carcinoma (NPC), establishes three types of latency that 
      facilitate its episomal genome persistence and evasion of host immune responses. 
      The innate inflammasome responses recognize the pathogen-associated molecular 
      patterns which lead into the association of a cytoplasmic sensor such as NLRP3 
      and AIM2 proteins or nuclear interferon-inducible protein 16 (IFI16) with adaptor 
      ASC protein (apoptosis-associated speck-like protein with a caspase recruitment 
      domain) and effector procaspase-1, resulting in active caspase-1 formation which 
      cleaves the proforms of inflammatory interleukin-1beta (IL-1beta), IL-18, and IL-33 
      cytokines. Whether inflammasome responses recognize and respond to EBV genome in 
      the nuclei was not known. We observed evidence of inflammasome activation, such 
      as the activation of caspase-1 and cleavage of pro-IL-1beta, -IL-18, and -IL-33, in 
      EBV latency I Raji cells, latency II NPC C666-1 cells, and latency III 
      lymphoblastoid cell lines (LCL). Interaction between ASC with IFI16 but not with 
      AIM2 or NLRP3 was detected in all three latencies and during EBV infection of 
      primary human B cells. IFI16 and cleaved caspase-1, IL-1beta, IL-18, and IL-33 were 
      detected in the exosomes from Raji cells and LCL. Though EBV nuclear antigen 1 
      (EBNA1) and EBV-encoded small RNAs (EBERs) are common to all forms of EBV 
      latency, caspase-1 cleavage was not detected in cells expressing EBNA1 alone, and 
      blocking EBER transcription did not inhibit caspase-1 cleavage. In fluorescence 
      in situ hybridization (FISH) analysis, IFI16 colocalized with the EBV genome in 
      LCL and Raji cell nuclei. These studies demonstrated that constant sensing of 
      latent EBV genome by IFI16 in all types of latency results in the constitutive 
      induction of the inflammasome and IL-1beta, IL-18, and IL-33 maturation.
FAU - Ansari, Mairaj Ahmed
AU  - Ansari MA
AD  - H. M. Bligh Cancer Research Laboratories, Department of Microbiology and 
      Immunology, Chicago Medical School, Rosalind Franklin University of Medicine and 
      Science, North Chicago, Illinois USA.
FAU - Singh, Vivek Vikram
AU  - Singh VV
FAU - Dutta, Sujoy
AU  - Dutta S
FAU - Veettil, Mohanan Valiya
AU  - Veettil MV
FAU - Dutta, Dipanjan
AU  - Dutta D
FAU - Chikoti, Leela
AU  - Chikoti L
FAU - Lu, Jie
AU  - Lu J
FAU - Everly, David
AU  - Everly D
FAU - Chandran, Bala
AU  - Chandran B
LA  - eng
GR  - CA 168472/CA/NCI NIH HHS/United States
GR  - R01 CA075911/CA/NCI NIH HHS/United States
GR  - R01 CA180758/CA/NCI NIH HHS/United States
GR  - R01 CA168472/CA/NCI NIH HHS/United States
GR  - CA 075911/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130529
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (IL33 protein, human)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-33)
RN  - 0 (Interleukins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 148998-64-5 (IFI16 protein, human)
SB  - IM
EIN - J Virol. 2017 Nov 14;91(23 ):. PMID: 29138330
MH  - B-Lymphocytes/*immunology/virology
MH  - Cells, Cultured
MH  - Epithelial Cells/*immunology/virology
MH  - Herpesvirus 4, Human/*immunology/*physiology
MH  - Humans
MH  - Hydrolysis
MH  - Inflammasomes/*metabolism
MH  - Interleukin-18/metabolism
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-33
MH  - Interleukins/metabolism
MH  - Nuclear Proteins/*metabolism
MH  - Phosphoproteins/*metabolism
MH  - *Virus Latency
PMC - PMC3719826
EDAT- 2013/05/31 06:00
MHDA- 2013/09/21 06:00
PMCR- 2014/02/01
CRDT- 2013/05/31 06:00
PHST- 2013/05/31 06:00 [entrez]
PHST- 2013/05/31 06:00 [pubmed]
PHST- 2013/09/21 06:00 [medline]
PHST- 2014/02/01 00:00 [pmc-release]
AID - JVI.00805-13 [pii]
AID - 00805-13 [pii]
AID - 10.1128/JVI.00805-13 [doi]
PST - ppublish
SO  - J Virol. 2013 Aug;87(15):8606-23. doi: 10.1128/JVI.00805-13. Epub 2013 May 29.