PMID- 23721784 OWN - NLM STAT- MEDLINE DCOM- 20140306 LR - 20130729 IS - 1872-7972 (Electronic) IS - 0304-3940 (Linking) VI - 549 DP - 2013 Aug 9 TI - Electroconvulsive stimulation alters levels of BDNF-associated microRNAs. PG - 125-9 LID - S0304-3940(13)00470-9 [pii] LID - 10.1016/j.neulet.2013.05.035 [doi] AB - Electroconvulsive therapy (ECT) is the most effective antidepressant treatment available yet its mechanism of action remains incompletely understood. Electroconvulsive stimulation (ECS), a rat model of electroconvulsive therapy, induces expression of brain derived neurotrophic factor (BDNF) within the brain but the precise means by which this occurs and by which it contributes to the antidepressant effects of ECS are unknown. MicroRNAs (miRNAs), a class of endogenous small non-coding RNA species, may play a role as BDNF can both regulate and be regulated by miRNAs. We examined expression of BDNF-associated miRNAs in rat brain and blood following either acute (x1) or chronic (x10) ECS. Fourteen BDNF-associated miRNA species were identified for investigation using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). We found that levels of miRNA miR-212 were significantly increased in rat dentate gyrus following both acute and chronic ECS. MiR-212 levels also increased in whole blood following chronic ECS and this positively correlated with miR-212 levels in the dentate gyrus. Our results suggest that alterations in miRNA expression may be informative about the mechanism of action of ECS/ECT. CI - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved. FAU - Ryan, Karen M AU - Ryan KM AD - Trinity College Institute of Neuroscience, Trinity College Dublin, Dublin 2, Ireland. FAU - O'Donovan, Sinead M AU - O'Donovan SM FAU - McLoughlin, Declan M AU - McLoughlin DM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130527 PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (MIRN212 microRNA, rat) RN - 0 (MicroRNAs) SB - IM MH - Animals MH - Brain/*metabolism MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism MH - Electroconvulsive Therapy MH - *Electroshock MH - Male MH - MicroRNAs/genetics/*metabolism MH - Rats MH - Rats, Sprague-Dawley OTO - NOTNLM OT - 3' untranslated region OT - 3'UTR OT - BDNF OT - Depression OT - ECS OT - ECT OT - MDD OT - RISC OT - RNA-induced silencing complex OT - SERT OT - SSRI OT - brain derived neurotrophic factor OT - electroconvulsive stimulation OT - electroconvulsive therapy OT - major depressive disorder OT - miRNAs OT - microRNA OT - microRNAs OT - qRT-PCR OT - quantitative reverse transcriptase polymerase chain reaction OT - selective serotonin reuptake inhibitor OT - serotonin transporter EDAT- 2013/06/01 06:00 MHDA- 2014/03/07 06:00 CRDT- 2013/06/01 06:00 PHST- 2013/04/09 00:00 [received] PHST- 2013/05/15 00:00 [accepted] PHST- 2013/06/01 06:00 [entrez] PHST- 2013/06/01 06:00 [pubmed] PHST- 2014/03/07 06:00 [medline] AID - S0304-3940(13)00470-9 [pii] AID - 10.1016/j.neulet.2013.05.035 [doi] PST - ppublish SO - Neurosci Lett. 2013 Aug 9;549:125-9. doi: 10.1016/j.neulet.2013.05.035. Epub 2013 May 27.