PMID- 23722048
OWN - NLM
STAT- MEDLINE
DCOM- 20140213
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 49
DP  - 2013 Nov 15
TI  - A novel thermometric biosensor for fast surveillance of beta-lactamase activity in 
      milk.
PG  - 99-104
LID - S0956-5663(13)00319-9 [pii]
LID - 10.1016/j.bios.2013.05.005 [doi]
AB  - Regulatory restrictions on antibiotic residues in dairy products have resulted in 
      the illegal addition of beta-lactamase to lower antibiotic levels in milk in China. 
      Here we demonstrate a fast, sensitive and convenient method based on enzyme 
      thermistor (ET) for the surveillance of beta-lactamase in milk. A fixed amount of 
      penicillin G, which is a specific substrate of beta-lactamase, was incubated with 
      the milk sample, and an aliquot of the mixture was directly injected into the ET 
      system to give a temperature change corresponding to the remained penicillin G. 
      The amount of beta-lactamase present in sample was deduced by the penicillin G 
      consumed during incubation. This method was successfully applied to quantify 
      beta-lactamase in milk with the linear range of 1.1-20 UmL(-1) and the detection 
      limit of 1.1 UmL(-1). The recoveries ranged from 93% to 105%, with relative 
      standard deviations (RSDs) below 8%. The stability of the column equipped in ET 
      was also studied, and only 5% decrease of activity was observed after 60 days of 
      use. Compared with the conventional culture-based assay, the advantages of high 
      throughput, timesaving and accurate quantification have made this method an ideal 
      alternative for routine use.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Zhou, Shuang
AU  - Zhou S
AD  - China National Center for Food Safety Risk Assessment, Key Laboratory of Food 
      Safety Risk Assessment, Ministry of Health, Beijing 100021, PR China. 
      szhoupku@gmail.com
FAU - Zhao, Yunfeng
AU  - Zhao Y
FAU - Mecklenburg, Michael
AU  - Mecklenburg M
FAU - Yang, Dajin
AU  - Yang D
FAU - Xie, Bin
AU  - Xie B
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130509
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Anti-Bacterial Agents)
RN  - EC 3.5.2.6 (beta-Lactamases)
RN  - Q42T66VG0C (Penicillin G)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/metabolism
MH  - Biosensing Techniques/*methods
MH  - Enzyme Assays/methods
MH  - Limit of Detection
MH  - Milk/chemistry/*enzymology
MH  - Penicillin G/metabolism
MH  - beta-Lactamases/*analysis/*metabolism
OTO - NOTNLM
OT  - Antibiotics
OT  - Enzyme immobilization
OT  - Enzyme thermistor
OT  - Milk
OT  - beta-lactamase
EDAT- 2013/06/01 06:00
MHDA- 2014/02/14 06:00
CRDT- 2013/06/01 06:00
PHST- 2013/02/21 00:00 [received]
PHST- 2013/04/12 00:00 [revised]
PHST- 2013/05/02 00:00 [accepted]
PHST- 2013/06/01 06:00 [entrez]
PHST- 2013/06/01 06:00 [pubmed]
PHST- 2014/02/14 06:00 [medline]
AID - S0956-5663(13)00319-9 [pii]
AID - 10.1016/j.bios.2013.05.005 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2013 Nov 15;49:99-104. doi: 10.1016/j.bios.2013.05.005. Epub 
      2013 May 9.